筛选出对植物乳杆菌RS66CD生物膜形成促进效果最显著的金属离子,确定生物膜形成最适培养条件,探究生物膜形成后对温度、pH、盐浓度和胆盐的耐受性。在培养基里添加Na+、Mn2+、Ca2+、Mg2+和Fe3+ 5种金属离子后,利用96孔法及扫描电镜测定生物膜形成量,选出最佳金属离子。对其添加量、培养温度和培养时间下生物膜形成的规律进行研究,确定了生物膜形成的最适条件。结果表明,Na+对生物膜形成具有明显促进效果。在NaCl质量浓度为53 g/L,40 ℃培养24 h时,生物膜的形成量最大。形成生物膜后的菌株对温度、pH、盐浓度和胆盐的耐受性均有明显提升。Na+可以促进植物乳杆菌生物膜的形成,在工业生产中,可以通过形成生物膜,使微生物在不良环境中保持活性。
The most effective metal ion was selected and the optimal culture condition was determined for RS66CD biofilm formation. The tolerance of biofilm to temperature, pH, salt concentration and bile salt was also studied. After adding Na+, Mn2+, Ca2+, Mg2+ and Fe3+, biofilm formation was determined by the 96-well method and SEM and then the best metal ion was selected. Under different ion concentrations, growth temperatures, and growth times, the biofilm formation was also studied to identify the optimal condition. The result showed that NaCl concentration of 53 g/L, the culture time of 24 h, and the culture temperature of 40 ℃ were the optimal condition for RS66CD biofilm formation. The environmental tolerance of strains after biofilm formation was improved. Na+ can promote the biofilm formation of Lactobacillus plantarum. In industrial production, microorganisms can be kept active by biofilms under stress condition.
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