该研究采用多位点序列分型方法将马乳酒样乳杆菌ZW3与其他同种或近源乳杆菌菌株进行细致的分类鉴定,同时确定马乳酒样乳杆菌ZW3特定的生物遗传标签,使用多位点序列分型方法对马乳酒样乳杆菌ZW3菌株与9株同种或近源乳杆菌一起进行多位点序列分型研究。该研究选取了7个管家基因(rpoA、Hsp60、LeuS、rpsB、glyK、pyrG、fusA)对这10株同种或近源乳杆菌菌株进行分型,最终得到8个序列型,马乳酒样乳杆菌ZW3单独拥有一个序列型ST8。这7个基因中,除pyrG外其余6个基因存在连锁不平衡现象,发生过轻微的基因重组现象,发生的变异通常为中性选择。马乳酒样乳杆菌ZW3与马乳酒样乳杆菌an3、shan3、shan4、shan5之间的遗传距离很近,与另外5株菌之间的遗传距离较远。该研究中选取的这7个管家基因可以将马乳酒样乳杆菌ZW3与其他同种或近源乳杆菌菌株进行区别,马乳酒样乳杆菌ZW3菌株的识别有助于在商业制品中确定其特定的益生功能。
For the specific bio-genetic label of L. kefiranofaciens ZW3, multiloci sequence typing (MLST) was used to classify and identify the strain with nine homologous or closely related strains. Seven housekeeping genes (rpoA, Hsp60, LeuS, rpsB, glyK, pyrG, fusA) were used for classification and eight sequence types (ST) were obtained, in which ST8 was found only in L. kefiranofaciens ZW3. Linkage disequilibrium and slight genetic recombination phenomenon, mostly neutral choice, were revealed in 6 of the 7 genes except for pyrG. L. kefiranofaciens ZW3 exhibited closer genetic distance with L. kefiranofaciens an3, L. kefiranofaciens shan3, L. kefiranofaciens shan4 and L. kefiranofaciens shan5 than other five strains. Identification of L. kefiranofaciens ZW3 lays a foundation for the research of its specific probiotic function in commercial products.
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