榨取甜橙和宽皮柑橘各20多个品种的果汁,离心后所得上清液和果肉沉淀分别采用C18固相萃取和乙腈提取其中的多甲氧基黄酮(polymethoxyflavoned,PMFs),合并后利用高效液相色谱-二极管阵列-荧光检测串联法(high-performance liquid chromatography-diode array detection-fluorescence detection, HPLC-DAD-FLD)做定性和定量分析。以8种PMFs为变量,通过主成分分析,建立了两类果汁的鉴别模型,开发了一种经济便利的鉴别甜橙汁中是否过量掺入宽皮柑橘汁的方法。该模型对橙汁(长叶香橙汁)中掺入的宽皮柑橘汁(椪柑汁)的最低检出体积分数为10%。该方法达到国际通行标准中对橙汁中其他柑橘果汁最大添加量的检出要求,对鉴别甜橙汁纯度具有良好的效果和实际应用价值。
周琦
,
谈安群
,
易鑫
,
李贵节
,
程玉娇
,
谭祥
,
ROUSEFF RUSSELL LEE
,
吴厚玖
,
翟雨淋
,
廖洪波
,
王华
. 基于柑橘汁多甲氧基黄酮特征成分鉴别橙汁中宽皮桔汁[J]. 食品与发酵工业, 2019
, 45(17)
: 227
-233
.
DOI: 10.13995/j.cnki.11-1802/ts.020640
An economical and convenient method for identifying mandarin juice over-blended into sweet orange juice was developed. Sweet orange and mandarin juices were extracted from more than 20 varieties for each group. After centrifugation of the juices, the supernatant and fruit pulp precipitate were obtained and their polymethoxyflavones (PMFs) were separately extracted by C18 solid phase extraction and acetonitrile. The extracts were combined and PMFs were qualitatively and quantitatively analyzed by high-performance liquid chromatography-diode array detection- fluorescence detection (HPLC-DAD-FLD). The identification model to discriminate two kinds of juice was established by principal component analysis (PCA) using the 8 PMFs as variables. This model was capable of detecting a minimum 10% volume fraction of mandarin juice (using Ponkan juice as an example) added into orange juice (using Long-leaf orange juice as an example). This method meets the international standard for detecting the maximum amount of adulterated juices into orange juice and also shows good efficacy and important practical value to identify the purity of sweet orange juices.
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