以凡纳滨对虾虾头为研究对象,探究UV-C辐照对其主要内源酶的影响。用功率为30 W的UV-C紫外灯以20 cm高度、辐照20 min,处理虾头均浆液,检测内源酶酶活,分析比较辐照前后温度、pH对其内源酶酶活的影响规律。从虾头中检测出酸性及碱性蛋白酶、脂肪酶、几丁质酶和多酚氧化酶等酶活性;UV-C辐照前后,脂肪酶、几丁质酶的最适pH有所变化,脂肪酶最适pH由10变为6~10,几丁质酶最适pH由5变为6左右;各主要内源酶最适温度无明显变化;UV-C辐照后,在最适条件下酸性蛋白酶酶活升高23.99%,碱性蛋白酶升高37.49%,脂肪酶升高21.37%,几丁质酶升高37.70%,多酚氧化酶升高46.52%。结果显示,UV-C辐照对凡纳滨对虾虾头酸性及碱性蛋白酶、脂肪酶、几丁质酶和多酚氧化酶等主要内源酶均有激活作用。
The effects of UV-C irradiation on the enzymatic properties of main endogenous enzymes in shrimp head of Litopenaeus vannamei were studied. The homogenized shrimp head was irradiated with 30 W UV-C ultraviolet lamp at a height of 20 cm for 20 min. The effects of temperature and pH on the activities of endogenous enzymes before and after irradiation were analyzed and compared. The optimum pH of lipase and chitinase changed before and after UV-C irradiation, as the optimum pH of lipase changed from 10 to 6-10, and the optimum pH of chitinase changed from 5 to 6. The optimum temperature of the main endogenous enzymes did not change significantly. After UV-C irradiation and under the optimum conditions, the activities of acid protease, alkaline protease, lipase, chitinase and PPO increased by 23.99%, 37.49%, 21.37%, 37.70% and 46.52%, respectively. Therefore, it was concluded that UV-C irradiation can activate the main endogenous enzymes in Litopenaeus vannamei head.
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