以甘肃河西走廊产区‘贵人香’冰白葡萄酒为试材,研究大豆蛋白、酪蛋白、聚乙烯吡咯烷酮(polyvinyl pyrrolidone, PVPP)、皂土和明胶5种不同类型下胶澄清剂对酒样澄清度、理化指标、香气成分和感官品质的影响。结果表明,下胶处理后冰白葡萄酒的透光率显著提高,色度降低(4.26%~16.47%),酒样中挥发酸(1.96%~3.92%)、总糖(0.7%~9.83%)、蛋白质(0~8.33%)和总酚(6.25%~10.42%)含量也有所降低;采用顶空固相微萃取结合气相色谱-质谱(heaspace solid-phase micro-extraction and gaschromatography-mass spectrometry, HS-SPME-GC/MS)技术检测其挥发性成分,共检测出67种挥发性香气化合物,包括24种酯类、20种醇类、9种酸类、6种羰基类、5种萜烯类和3种其它类香气物质,经下胶澄清处理后酒样中挥发性香气物质的种类(7.46%~19.40%)和含量(4.60%~26.52%)均有所下降,其中大豆蛋白处理组对挥发性香气物质的影响较小,感官品质较好,可用于‘贵人香’冰白葡萄酒的下胶澄清。
‘Italian Riesling’ ice wine produced in Gansu Hexi Corridor was selected to study the effects of five different clarifying agents (soy protein, casein, PVPP, bentonite and gelatin) on its clarity degree, physicochemical parameters, volatile composition and sensory quality. The results showed that after clarifying process, the transmittance of ice wine improved significantly, while the contents of volatile acids (1.96%-3.92%), total sugar (0.7%-9.83%), protein (0-8.33%) and phenols (6.25%-10.42%) as well as color intensity (4.26%-16.47%) reduced in different levels. Gas Chromatography-Mass Spectrometry (GC-MS) combined with headspace solid-phase microextraction (HS-SPME) were used to detect the volatile compounds. A total of 67 volatile constituents were identified and quantified, including 24 esters, 20 alcohols, 9 acids,6 carbonyls, 5 terpenes and 3 other compounds. As expected, the varieties (7.46%-19.40%) and contents (4.60%-26.52%) of aroma compounds of ice wine were declined after clarification. Among them, samples treated by soy protein remained higher contents of flavor components and excellent sensory evaluation, which indicated that soy protein could be used in for ‘Italian Riesling’ ice wine clarification.
[1] IGOR L, SANJA R, NIKOLA G, et al.Changes in physico-chemical and volatile aroma compound composition of Gewürztraminer wine as a result of late and ice harvest[J]. Food Chemistry, 2016, 196(2): 1 048-1 057.
[2] TIAN R R, LI G, WAN S B, et al.Comparative study of 11 phenolic acids and five flavan-3-ols in cv. Vidal: impact of natural icewine making versus concentration technology[J]. Australian Journal of Grape & Wine Research, 2010, 15(3): 216-222.
[2] BUČKOVÁ M, PUŠKÁROVÁ A, ŽENIŠOVÁ K, et al. Novel insights into microbial community dynamics during the fermentation of Central European ice wine[J]. International Journal of Food Microbiology, 2017, 266: 42-51.
[4] WANG J, LI M, LI J X, et al.Chapter 10-Biotechnology of Ice Wine Production[M]. Netherlands: Advances in Biotechnology in the Food Industry-Elsevier, 2018: 267-301.
[5] 张建华. 多酚-溶菌酶-SO2复合体系在‘贵人香’冰白葡萄酒中的应用研究[D]. 兰州:甘肃农业大学, 2016.
[6] 黄卫东, 李景明, 王秀芹. 冰葡萄酒生产及其在我国的发展[J]. 农产品加工(学刊), 2005, (Z2): 56-61.
[7] 唐柯, 马玥, 徐岩, 等. 冰葡萄酒重要风味化合物嗅觉阈值的研究[J]. 食品与发酵工业, 2016, 42(1): 148-151.
[8] 李新榜, 张瑛莉, 范永峰. 葡萄酒澄清和稳定工艺理论与实践探讨[J].中外葡萄与葡萄酒, 2011(1):57-62.
[9] 李艳敏, 赵树欣. 不同酒类澄清剂的澄清机理与应用[J]. 中国酿造, 2008, 27(1): 1-5.
[10] MAURY C, SARNIMANCHADO P.Influence of Fining with Plant Proteins on Proanthocyanidin Composition of Red Wines[J]. American Journal of Enology & Viticulture, 2003, 54(2): 105-111.
[11] 孙喜房, 李春, 党艳艳, 等. 改型膨润土对红葡萄酒澄清过程中蛋白质和色素的影响[J]. 食品科学, 2006, 27(8): 148-151.
[12] 陈彦雄, 祝霞, 潘陆霞, 等. 不同澄清处理对赤霞珠干红葡萄酒澄清度和色度的影响[J]. 农产品加工(学刊), 2010(1): 19-21.
[13] COSME F, CAPÃO I, FILIPE-RIBEIRO L, et al. Evaluating potential alternatives to potassium caseinate for white wine fining: Effects on physicochemical and sensory characteristics[J]. LWT-Food Science and Technology, 2012, 46(2): 382-387.
[14] GRANATO T M, NASI A, FERRANTI P, et al.Fining white wine with plant proteins: effects of fining on proanthocyanidins and aroma components[J]. European Food Research and Technology, 2014, 238(2): 265-274.
[15] MIRA H, LEITE P, RICARDO-DA-SILVA J M, et al. Plant proteins in wine fining: influence on chemical and sensory characteristics[J]. Bulletin De Lo.i.v, 2006, 79: 277-296.
[16] 张艳, 谢春阳. 大豆蛋白在山葡萄酒澄清处理中的应用研究[J]. 当代生态农业, 2010(Z2): 140-143.
[17] 鲁榕榕,马腾臻,张波,等. 不同澄清剂对起泡葡萄酒原酒的澄清作用及对香气品质的影响[J]. 食品科学, 2018, 39(12): 146-153.
[18] 鲁榕榕, 马腾臻, 张波, 等. 大豆蛋白澄清剂对‘赤霞珠’干红葡萄酒品质的影响[J]. 食品与发酵工业, 2018, 44(3): 135-145.
[19] GB/T 15038—2006B/T 15038—2006. 葡萄酒、果酒通用分析方法[S]. 北京: 中国标准出版社, 2006.
[20] MARTINEZ-RODRIGUER A, CARRASCOSA A V, BARCENILLA J M, et al.Autolytic capacity and foam analysis as additional criteria for the selection of yeast strains for sparkling wine production[J]. Food Microbiology, 2001, 18(2): 183-191.
[21] BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72(s 1-2): 248-254.
[22] SINGLETON V L, ROSSI J A.Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents[J]. American journal of Enology and Viticulture, 1965, 16(3): 144-158.
[23] 陈霞, 李敏, 张波, 等. 扩展青霉对‘蛇龙珠’葡萄酒棒曲霉素及风味品质的影响[J]. 食品科学, 2016,37(20): 126-133.
[24] RIBEIRO T, FERNANDES C, NUNES F M, et al.Influence of the structural features of commercial mannoproteins in white wine protein stabilization and chemical and sensory properties[J]. Food Chemistry, 2014, 159(11): 47-54.
[25] COSME F, RICARDO-DA-SILVA J M, LAUREANO O. Interactions between protein fining agents and proanthocyanidins in white wine[J]. Food Chemistry, 2008, 106(2): 536-544.
[26] GUERRERO R F, SMITH P, BINDON K A.Application of insoluble fibers in the fining of wine phenolics[J]. Journal of Agricultural & Food Chemistry, 2013, 61(18): 4 424-4 432.
[27] CHEN K, HAN S Y, LI M, et al.Use of lysozyme and oligomeric proanthocyanidin to reduce sulfur dioxide and the evolution of volatile compounds in Italian Riesling ice wine during aging process[J]. Journal of Food Processing & Preservation, 2017, 41(1). DOI: 10.1111/jfpp.12755.
[28] 申静云, 刘沛通, 段长青, 等. 不同有孢汉逊酵母与酿酒酵母混合发酵对威代尔冰葡萄酒香气的影响[J]. 食品与发酵工业, 2017, 43(10): 16-23.
[29] SWIEGERS J H, BARTOWSKY E J, HENSCHKE P A, et al.Yeast and bacterial modulation of wine aroma and flavour[J]. Australian Journal of Grape & Wine Research, 2010, 11(2): 139-173.
[30] COMUZZO P, MARCONI M, ZANELLA G, et al.Pulsed electric field processing of white grapes (cv. Garganega): Effects on wine composition and volatile compounds[J]. Food Chemistry, 2018, 264:16-23.
[31] MELISSA S, CHARLESG E, CAROLYNF R.Impact of fining on chemical and sensory properties of Washington State Chardonnay and Gewurztraminer wines[J]. American Journal of Enology & Viticulture, 2010, 61(1): 31-41.
[32] VINCENZI S, PANIGHEL A, GAZZOLA D, et al.Study of combined effect of proteins and bentonite fining on the wine aroma loss[J]. Journal of Agricultural & Food Chemistry, 2015, 63(8): 2 314-2 320.