将蜂蜜中有害物质去除后再将其应用于食品加工或其他领域,可有效实现资源再利用。采用大孔树脂吸附技术,研究了树脂类型及工艺条件对脱除蜂蜜中5-羟甲基糠醛(5-hydroxymet hylfurfural, 5-HMF)的影响,并探究吸附前后蜂蜜理化指标及酚类组成的差异,以及对不同种类蜂蜜的适用性。结果表明,弱极性树脂适合脱除蜂蜜中5-HMF,其中LSI-3型树脂效果最佳;降低温度、增加树脂用量以及延长吸附时间有助于提高5-HMF脱除效果,在25 ℃、树脂用量44 g/kg(蜂蜜)、80 min优化吸附工艺条件下,LSI-3型树脂对5-HMF脱除率可达62.49%;另外,LSI-3型树脂吸附对蜂蜜电导率、pH和还原糖总量没有显著影响,但淀粉酶值略有降低,酚类成分则明显减少,该方法对脱除不同种类蜂蜜中5-HMF具有良好适用性。该研究为5-HMF含量超标的蜂蜜提供了一种资源再利用的新途径。
The removal of hazardous substances from honey is beneficial for the utilization in food processing or other fields, which will contribute to realize the resource reuse effectively. By virtue of macroporous resin adsorption technology, the effects of resin type and process conditions on the removal of 5-hydroxymethylfurfural (5-HMF) from 5-HMF positive honey sample were investigated, and the physicochemical indexes and phenolic ingredients of honey before and after resin adsorption were analyzed, as well as the macroporous resin applicability to different types of honey. The results showed that weak polar resin was suitable for removing 5-HMF from honey, among which LSI-3 resin had the best removal effect. Reducing temperature, increasing resin content and prolonging adsorption time contributed to 5-HMF removal, and the removal rate reached 62.49% under the optimum adsorption condition of 25 ℃, 44 g/kg resin content and 80 min for LSI-3 resin. In addition, LSI-3 resin adsorption did not significantly affect the conductivity, pH and total reducing sugar content of honey, but the amylase value decreased slightly, and phenolic components decreased obviously, showing a good applicability for removing 5-HMF from different kinds of honey. This provides a new approach for treatment of honey with high 5-HMF content.
[1] DA SILVA P M, GAUCHE C, GONZAGA L V, et al. Honey: chemical composition, stability and authenticity[J]. Food Chemistry, 2016, 196:309-323.
[2] KADRI S M, ZALUSKI R, ORSI R D O. Nutritional and mineral contents of honey extracted by centrifugation and pressed processes[J]. Food Chemistry, 2017, 218:237-241.
[3] KHAN S U, ANJUM S I, RAHMAN K, et al. Honey: Single food stuff comprises many drugs[J]. Saudi Journal of Biological Sciences, 2018, 25(2):320-325.
[4] ISMAIL, M M, HAMAD, M F, ELRAGHY, E M. Using goat's milk, barley flour, honey, and probiotic to manufacture of functional dairy product[J]. Probiotics and Antimicrobial Proteins, 2019, 10(4):677-691.
[5] ÖNÜR I, MISRA N N, BARBA F J, et al. Effects of ultrasound and high pressure on physicochemical properties and HMF formation in Turkish honey types[J]. Journal of Food Engineering, 2018, 219:129-136.
[6] 马天琛, 王倩,程妮,等. 热加工对蜂蜜质量影响的研究进展[J]. 食品与发酵工业,2019,45(14):245-249;255.
[7] AL-FARSI M, AL-BELUSHI S, AL-AMRI A, et al. Quality evaluation of omani honey[J]. Food Chemistry, 2018, 262:162-167.
[8] 仵淑红, 郭栋,孟大均. 蜂蜜中5-羟甲基糠醛的检测[J]. 首都食品与医药, 2016, 23(20):76-77.
[9] 连会. 蜂蜜中四环素和羟甲基糠醛去除研究[D]. 无锡:江南大学, 2007.
[10] 宋秀超. 东北黑蜜蜂成分分析、羟甲基糠醛去除及解抗晶技术研究[D]. 哈尔滨:哈尔滨商业大学, 2014.
[11] 樊勇勇,郑琦,顾金凤,等.大孔树脂吸附技术及其应用[J]. 化工设计通讯, 2017, 43(3):147-148;150.
[12] 刘清清, 阎怡竹,杨海霞,等. 大孔吸附树脂对蜂蜜中拟除虫菊酯脱除的热力学性质研究[J]. 食品与发酵工业, 2016, 31(23):57-61.
[13] 王静, 雷宏杰,岳珍珍,等. 大孔树脂对红枣汁中棒曲霉素的吸附动力学[J]. 农业工程学报, 2015, 42(12):285-291.
[14] 高振鹏, 张丹,刘瑞,等. 苹果汁中展青霉素的去除动力学及热力学研究[J]. 农业机械学报, 2015, 46(10):304-310.
[15] 张春燕, 吴美儒,周毅峰,等. 利用大孔树脂对莼菜多糖脱色的工艺优化[J]. 食品工业科技, 2018, 39(15):154-157.
[16] 中华人民共和国国家质量监督检验检疫总局. 蜂蜜中淀粉酶值的测定方法分光光度法:GB/T 18932.16—2003[S].
[17] 中华人民共和国国家质量监督检验检疫总局. 食品安全国家标准食品中还原糖的测:GB 5009.7—2016[S].
[18] HINNEBURG I, DORMAN H D, HILTUNEN R. Antioxidant activities of extracts from selected culinary herbs and spices[J]. Food Chemistry, 2016, 97:122-129.
[19] 贺琼, 何亮亮,康予馨,等. 高效液相色谱-电化学检测指纹图谱鉴别3种单花种蜂蜜花源[J]. 食品科学, 2017, 38(2): 290-295.
[20] 中华人民共和国国家质量监督检验检疫总局. 蜂蜜中羟甲基糠醛含量的测定方法液相色谱-紫外检测:GB/T 18932.18—2003[S].
[21] CHENG N, GAO H,DENG J J, et al. Removal of chloramphenicol by macroporous adsorption resins in honey: a novel approach on reutilization of antibiotics contaminated honey[J]. Journal of Food Science, 2012, 77(9):T169-T172.
[22] IDRIS S A M. Adsorption,kinetic and thermodynamic studies for manganese extraction from aqueous medium using mesoporous silica[J]. Journal of Colloid and Interface Science, 2015, 440:84-90.
[23] 雷海燕. 蜂蜜中甲硝唑和氧氟沙星的残留监测和脱除技术研究[D]. 西安:西北大学, 2018.
[24] 孟范平, 马冬冬,姚瑞华. 苯乙烯型大孔树脂对海水中那个微量有机磷农药的吸附性能研究[J]. 中国海洋大学学报(自然科学版), 2006, 36(2):303-306.
[25] SALIM O, NADIA A, MARIA G. Characterization of phenolic compounds in Algerian honeys by RP-HPLC coupled to electrospray time-of-flight mass spectrometry[J]. LWT-Food Science and Technology, 2017, 85:460-469.
[26] ZHAO H A, CHENG N, WANG Q, et al. Effects of honey-extracted polyphenols on serum antioxidant capacity and metabolic phenotype in rats[J]. Food & Function, 2019, 10(5):2 347-2 358.
