该文建立了花生油中黄曲霉毒素B1(Aflatoxin B1,AFB1)快速准确的检测方法。利用交流电动作用对AFB1进行加速和富集,然后在敏感元件微芯片上与固定的抗AFB1单克隆抗体发生免疫反应从而导致电容规律性变化,构建AFB1免疫传感器。结果表明,该方法可在30 s内完成加样、孵育和检测,实现对食用花生油中AFB1的高灵敏度定量分析。该方法针对测试液中AFB1浓度的线性范围为10-6～10-2μg/mL(R2=0.993 6);相对标准偏差(n=5)为6.63%~15.60%,最低检出限为2.01×10-7μg/mL(3 σ/S),定量限为2.35×10-7μg/mL(10 σ/S),平均加标回收率为85.20%～97.70%,对AFB2、AFM1、AFG1、OTA、DON、ZEN等可能共存毒素具有抗干扰性。
This study was conducted to develop a rapid and accurate method for Aflatoxin B1 (AFB1) detection in peanut oil. For accelerating the reaction, AFB1 was enriched via AC electrokinetics, while the anti-AFB1 monoclonal antibody was immobilized onto a microchip which was acted as a sensitive component. Subsequently, the enriched AFB1 reacted with the antibodies, leading to the change of capacitance regularity. Based on the above principle, the AFB1 immunosensor was finally created to achieve high sensitive and quantitative analysis of AFB1 in edible peanut oil only within 30 s including sampling, incubating and testing. For this method, in test solution, linear range and relative standard deviation (n=5) were 10-6-10-2 μg/mL (R2=0.993 6) and 6.63%-15.60%, respectively. The limit of detection and limit of quantitation reached 2.01×10-7 μg/mL (3 σ/S) and 2.35×10-7 μg/mL (10 σ/S), respectively, with the average recovery of standard addition in peanut oil of 85.20%-97.7%. Besides, this method showed the anti-interference ability to other common toxins in food, such as AFB2, AFM1, AFG1, OTA, DON, ZEN, etc. In conclusion, this method is accurate, rapid, and highly sensitive to be used for the determination of trace amount AFB1 in peanut oil.
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