食品与发酵工业 >

2020 , Vol. 46 >Issue 13: 127 - 132

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023436

研究报告

植物乳杆菌CCFM8724对致龋双菌生物膜的抑制作用

  • 秦苏佳 ,
  • 徐晚晴 ,
  • 张秋香 ,
  • 赵建新 ,
  • 张灏 ,
  • 陈卫
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  • (江南大学 食品学院,江苏 无锡,214122)
硕士研究生(张秋香副教授为通讯作者,E-mail:zhangqx@jiangnan.edu.cn)

收稿日期: 2020-01-20

  网络出版日期: 2020-08-04

基金资助

国家重点研发计划资助项目( 2017YFD0400600)

收起

Inhibitory effect of Lactobacillus plantarum CCFM8724 oncaries-causing dual biofilms

  • QIN Sujia ,
  • XU Wanqing ,
  • ZHANG Qiuxiang ,
  • ZHAO Jianxin ,
  • ZHANG Hao ,
  • CHEN Wei
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  • (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

Received date: 2020-01-20

  Online published: 2020-08-04

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摘要

变异链球菌(Streptococcus mutans)生物膜中白色念珠菌(Candida albicans)的存在会增强生物膜的致龋毒力,并导致与幼儿龋病(early childhood caries, ECC)相似的猖獗性龋病的发生。为验证植物乳杆菌(Lactobacillus plantarum)CCFM8724对变异链球菌和白色念珠菌双菌生物膜的体外抑制效果,在双菌生物膜形成的不同时期进行介导,采用结晶紫染色法、蒽酮硫酸法、平板计数法对介导后的生物膜进行评估,并用激光共聚集和扫描电镜对生物膜活死菌菌体和结构进行观察,最后测定CCFM8724对唾液包被羟基磷灰石(saliva-coated hydroxyapatite discs,SHA)的脱钙量和它对溶菌酶的耐受能力。结果表明,植物乳杆菌CCFM8724能显著降低双菌生物膜量、水不溶性胞外多糖的产量以及致病菌活菌数,并破坏生物膜的活性和结构。对SHA的脱钙情况有所缓解,对溶菌酶耐受质量浓度为1.6~2.0 mg/mL,说明CCFM8724具有成为防龋口腔益生菌的应用潜力。

关键词： 龋齿; 变异链球菌; 白色念珠菌; 致龋生物膜; 牙菌斑; 益生菌

本文引用格式

秦苏佳 , 徐晚晴 , 张秋香 , 赵建新 , 张灏 , 陈卫 . 植物乳杆菌CCFM8724对致龋双菌生物膜的抑制作用[J]. 食品与发酵工业, 2020 , 46(13) : 127 -132 . DOI: 10.13995/j.cnki.11-1802/ts.023436

Abstract

The presence of Candida albicans in dental plaque with Streptococcus mutans enhances the virulence leading to the onset of rampant caries which is similar to early childhood caries (ECC). In order to verify the inhibitory effect of Lactobacillus plantarum CCFM8724 on the dual biofilm which infected by S. mutans and C. albicans at different time points in vitro, the treated biofilms were assessed for crystal violet staining, anthrone-sulfuric method and colony forming unit (CFU) counting. The live/dead bacteria and structure of biofilm were observed by laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Finally, the decalcification of saliva-coated hydroxyapatite discs (SHA) after culture with CCFM8724 and the tolerance of lysozyme were determined. The results showed that L. plantarum CCFM8724 could significantly reduce the biomass of dual biofilm, the production of water-insoluble extracellular polysaccharide (EPS) and the CFU of pathogens. It also reduced the viability of biofilm and destroyed the structure of biofilm. Moreover, it alleviated the decalcification of SHA with the tolerance concentration to lysozyme 1.6-2.0 mg/mL. It is suggested that CCFM8724 has the potential to be used as an oral probiotic for caries prevention.

Key words： caries; Streptococcus mutans; Candida albicans; cariogenic biofilm; dental plaque; probiotics

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