变异链球菌(Streptococcus mutans)生物膜中白色念珠菌(Candida albicans)的存在会增强生物膜的致龋毒力,并导致与幼儿龋病(early childhood caries, ECC)相似的猖獗性龋病的发生。为验证植物乳杆菌(Lactobacillus plantarum)CCFM8724对变异链球菌和白色念珠菌双菌生物膜的体外抑制效果,在双菌生物膜形成的不同时期进行介导,采用结晶紫染色法、蒽酮硫酸法、平板计数法对介导后的生物膜进行评估,并用激光共聚集和扫描电镜对生物膜活死菌菌体和结构进行观察,最后测定CCFM8724对唾液包被羟基磷灰石(saliva-coated hydroxyapatite discs,SHA)的脱钙量和它对溶菌酶的耐受能力。结果表明,植物乳杆菌CCFM8724能显著降低双菌生物膜量、水不溶性胞外多糖的产量以及致病菌活菌数,并破坏生物膜的活性和结构。对SHA的脱钙情况有所缓解,对溶菌酶耐受质量浓度为1.6~2.0 mg/mL,说明CCFM8724具有成为防龋口腔益生菌的应用潜力。
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