鲜切黄瓜在国内外具有很大的消费市场,但在贮藏期间极易受微生物污染而腐败变质。变形假单胞菌是低温贮藏条件下导致鲜切黄瓜腐败的主要微生物之一。该研究以变形假单胞菌为实验菌株,接种至鲜切黄瓜表面后,探讨3%O2、7%CO2气调箱贮藏对菌株生长和侵染情况,对黄瓜细胞壁降解和细胞壁降解引起的生理生化变化的影响。结果表明,与对照组相比,3%O2、7%CO2气调贮藏抑制了变形假单胞菌的生长,保持了细胞壁的完整性,延缓了原果胶的降解,降低了纤维素酶活性,抑制了β-半乳糖苷酶活性的增加和可溶性果胶的产生,从而保持了硬度,延缓了丙二醛含量和相对电导率的增加,维持了鲜切黄瓜的外观和色泽。总之,3%O2、7% CO2气调箱贮藏抑制了由变形假单胞菌导致的鲜切黄瓜细胞壁的降解,延缓了腐败变质。
Fresh-cut cucumber has a large market in the world, but they are prone to spoilage due to the contamination of the microorganisms during storage. Pseudomonas plecoglossicida is one of the main spoilage bacteria that cause the deterioration of fresh cut cucumber at low temperature storage. Fresh-cut cucumber was inoculated with Pseudomonas plecoglossicida, then stored at 3% O2, 7% CO2 controlled atmosphere for study of the growth and the infection of Pseudomonas plecoglossicida, the degradation of cell wall and physiological and biochemical changes caused by cell wall degradation of fresh-cut cucumber. The results showed that 3% O2 and 7% CO2 controlled atmosphere storage inhibited the growth of Pseudomonas plecoglossicida, kept the integrity of cell wall, delayed the degradation of protopectin, reduced the cellulase activity, inhibited the increase of β-galactosidase activity and the production of soluble pectin. And thus maintained firmness and delayed the increase of malondialdehyde content and relative conductivity, which also helped to maintain the appearance and color of fresh-cut cucumber. In conclusion, 3% O2 and 7% CO2 controlled atmosphere storage inhibited the cell wall degradation caused by Pseudomonas plecoglossicida and delayed the spoilage of fresh-cut cucumber.
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