该文以灭菌率及生长趋势为评价指标,研究了样品水分含量、微波功率、处理时间对蜡样芽孢杆菌灭菌效果的影响,并通过正交试验探索了微波处理对小麦粉中蜡样芽孢杆菌的最优灭菌条件。结果表明,随样品水分含量升高微波处理对蜡样芽孢杆菌的杀灭率呈现先升高后降低的趋势,且在样品水分含量为15%时,杀灭率最高;微波对蜡样芽孢杆菌的杀灭率随处理时间和微波功率的增加而提高,当微波功率达到581 W时,能够将小麦粉中蜡样芽孢杆菌含量降低到103CFU/g。正交试验结果表明,在处理时间7.5 min,微波功率700 W,样品水分含量15%的条件下,微波处理对蜡样芽孢杆菌的灭菌效果最佳,杀灭率可高达99%。
In order to obtain the optimal sterilization conditions of Bacillus cereus in wheat flour by microwave treatment,the effect of microwave time, microwave power and initial moisture content of sample on the sterilization effect of Bacillus cereus in wheat flour were investigated. And the sterilization rate and growth trend were evaluated as the indexes of sterilization effect. The sterilization rate of Bacillus cereus increased first and then decreased when the moisture content increased from 13% to 17%, and the sterilization rate was the highest when the moisture content was 15%. Moreover, the microwave sterilization rate of Bacillus cereus was increased with the increase of treatment time and microwave power. When the microwave treatment power higher than 581W, the content of Bacillus cereus in wheat flour reduced to 103 CFU/g. Different microwave treatment conditions lead to different growth trends of Bacillus cereus. The orthogonal test results showed that the sterilization rate was 99% at microwave power of 700 W, moisture content of 15% and treated for 7.5 min.
[1] AGATA N, OHTA M, YOKOYAMA K. Production of Bacillus cereus emetic toxin (cereulide) in various foods[J]. International Journal of Food Microbiology, 2002, 73: 23-27.
[2] PEI XIaoyan, YANG Shuruan, ZHAN Li, et al. Prevalence of Bacillus cereus in powdered infant and powdered follow-up formula in China[J]. Food Control, 2018, 93: 101-105.
[3] 柳增善.食品病原微生物学[M].北京:中国轻工业出版社,2007:72-73.
[4] 刘慧.现代食品微生物学[M].北京:中国轻工业出版社, 2011:130-137.
[5] OSIMANI A, AQUILANTI L. Clementi Bacillus cereus foodborne outbreaks in mass catering[J]. International Journal of Hospitality Management, 2018, 72: 145-153.
[6] 张林玉,郭艳明,伍晓聪,等.超高压处理对腌渍红椒灭菌效果及品质的影响[J].食品与发酵工业,2017,43(8):190-196.
[7] ZENG Shaoxiao, CHEN Bingyan, ZENG Hongliang, et al. Effect of microwave irradiation on the physicochemical and digestive properties of Lotus seed starch[J]. Agricultural And Food Chemistry, 2016, 64: 2 442-2 449.
[8] 郭全友,王晓晋,姜朝军.微波杀灭虾源地衣芽孢杆菌孢子特性及效果[J].农业工程学报,2018,34(21):281-287.
[9] KIM J E, CHOI H S, LEE D U. Effects of processing parameters on the inactivation of Bacillus cereus spores on red pepper (Capsicum annum L.) flakes by microwave-combined cold plasma treatment[J]. International Journal of Food Microbiology, 2017, 263: 61-66.
[10] 张黎骅,张文.响应面法优化酒糟微波间歇干燥工艺[J].农业工程学报,2011,27(3):369-374.
[11] 周小伟,刘晓丽,吴克刚,等.面粉微波处理结合植物精油对鲜湿面保鲜品质的影响研究[J].粮食与油脂,2016,29(11):62-65.
[12] 邹忠义,孙建利,冯志,等.微波处理对脱氧雪腐镰刀菌烯醇T-2毒素的去除作用[J].食品工业,2017,38(5):32-35.
[13] 王胜录,万忠民,陈培栋.高功率微波对稻谷中储粮害虫致死效果研究[J].粮食科技与经济,2017,42(5):49-54.
[14] AUKSORNSRIA T, TANG Juming, TANG Zhongwei, et al. Dielectric properties of rice model food systems relevant to microwave sterilization process[J]. Innovative Food Science and Emerging Technologies, 2018, 45: 98-105.
[15] 唐彬,靳苗苗,张洪翠,等.微波处理及其非热效应对麻辣鸡块品质的影响[J].食品与发酵工业,2018,44(12):200-207.
[16] MELIS V, BESTEN D, GROOT N, et al. Quantification of the impact of single and multiple mild stresses on outgrowth heterogeneity of Bacillus cereus spores[J]. International Journal of Food Microbiology, 2014, 177: 57-62.
[17] SONI A, OEY I, SILCOCK P, et al. Differential gene expression for investigation of the effect of germinants and heat activation to induce germination in Bacillus cereus spores[J]. Food Research International, 2019, 119: 462-468.
[18] RAJKOVIC A, KLJAJIC M, SMIGIC N, et al. Toxin producing Bacillus cereus persist in ready-to-reheat spaghetti Bolognese mainly in vegetative state[J]. International Journal of Food Microbiology, 2013, 167: 236-243.
[19] ABEE T, GROOT M N, TEMPELAARS M, et al. Germination and outgrowth of spores of Bacillus cereus group members: Diversity and role of germinant receptors[J]. Food Microbiology, 2011, 28: 199-208.
[20] VALERO M, LEONTIDIS S,FERNANDEZ P S, et al. Growth of Bacillus cereus in natural and acidified carrot substrates over the temperature range 5-30℃[J]. Food Microbiology, 2000, 17: 605-612.
[21] SONI A, OEY I, SILCOCK P, et al. Impact of temperature, nutrients, pH and cold storage on the germination, growth and resistance of Bacillus cereus spores in egg white[J]. Food Research International, 2018, 106: 394-403.
[22] 陈未,周伟伟,陈飞,等.蜡样芽孢杆菌芽孢萌发及其对常规灭菌方法抵抗力的研究[J].中国畜牧兽医, 2014,41(1):192-195.
[23] WANG Kaiqiang, LUO Shuizhong, ZHONG Xiyang, et al. Changes in chemical interactions and protein conformation during heat-induced wheat gluten gel formation[J]. Food Chemistry, 2017, 214: 393-399.
[24] LAMACCHIA C, LANDRISCINA L, D′AGNELLO P. Changes in wheat kernel proteins induced by microwave treatment [J]. Food Chemistry, 2016, 197: 634-640.
