研究报告

高转苷活性乳糖酶快速筛选方法的建立与初步应用

  • 赵继华 ,
  • 牛丹丹 ,
  • NOKUTHULA Peace Mchunu ,
  • 田康明 ,
  • 苗佳 ,
  • 王正祥
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  • 1(工业发酵微生物教育部重点实验室(天津科技大学 生物工程学院),天津,300457);
    2(天津科技大学 化工与材料学院,天津,300457);
    3(Biotechnology Platform, Agricultural Research Council, Pretoria 0001, South Africa)
硕士研究生(牛丹丹副研究员为通讯作者,E-mail:ddniu@tust.edu.cn)

收稿日期: 2020-02-20

  网络出版日期: 2020-05-19

基金资助

政府间国际合作重点项目(2018YFE0100400);国家自然科学基金(31601407);天津市高等学校创新团队建设规划(TD12-5009)

Establishment and preliminary application of a rapid screening method for lactase with high galactosyl transferase activity

  • ZHAO Jihua ,
  • NIU Dandan ,
  • NOKUTHULA Peace Mchunu ,
  • TIAN Kangming ,
  • MIAO Jia ,
  • WANG Zhengxiang
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  • 1(Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education (College of Biotechnology, Tianjin University of Science and Technology), Tianjin 300457, China);
    2(College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, China);
    3(Biotechnology Platform, Agricultural Research Council, Pretoria 0001, South Africa)

Received date: 2020-02-20

  Online published: 2020-05-19

摘要

具有良好半乳糖基转移酶活性的乳糖酶是工业制备低聚半乳糖(galacto-oligosaccharides, GOS)的主要酶制剂。通过筛选获得了以GOS为唯一碳源生长良好,在乳糖-葡萄糖-半乳糖中生长不良的长双歧杆菌B1172,确立了其GOS-生物量相关关系,进一步基于GOS-生物量相关关系建立起乳糖酶转苷活性的高通量筛选方法。运用此方法,从菌种库中筛选获得了5株具有较高转苷活性的乳糖酶产生菌株,可为后续此酶的克隆与分子进化奠定基础。

本文引用格式

赵继华 , 牛丹丹 , NOKUTHULA Peace Mchunu , 田康明 , 苗佳 , 王正祥 . 高转苷活性乳糖酶快速筛选方法的建立与初步应用[J]. 食品与发酵工业, 2020 , 46(7) : 17 -20 . DOI: 10.13995/j.cnki.11-1802/ts.023697

Abstract

The lactase with the high galactosyl transferase activity is desired for the galacto-oligosaccharides (GOS) preparation. Bifidobacterium longum B1172 growing well on GOS but not on lactose-glucose-galactose was selected and its GOS-biomass correlation was established. A high-throughput screening method for transgalactosylation activity was subsequently developed and successfully applied for screening transgalactosylase-producing strains from the culture collection. Five strains producing lactase with the remarkably transgalactosyl activity were selected, which lay a foundation for further cloning and molecular evolution of the enzyme.

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