通过人体粪便体外发酵试验和动物体内、体外发酵试验,研究魔芋葡甘聚糖(konjac glucomannan,KGM)和魔芋葡甘低聚糖(konjac oligo-glucomannan,KOGM)的肠道益生性对治疗乳糖不耐受症的可能性。在健康和乳糖不耐受受试者粪便菌群的体外发酵实验中,KGM和KOGM的添加降低了发酵液的pH,提高了短链脂肪酸(short chain fatty acids,SCFA)的产量(P<0.05)。灌胃小鼠KGM和KOGM 4 周后,结肠粪便的pH值降低,含水量和SCFA含量均显著增加(P<0.05)。KGM和KOGM对乳糖体外发酵特性的影响研究结果显示,KGM和KOGM组发酵液的pH值降低,SCFA含量和乳糖酶活性都得到明显升高(P<0.05)。实验结果表明,摄入KGM和KOGM可以为机体提供良好的酸性环境,有效改善肠道微生物菌群代谢产生SCFA,促进结肠发酵。KGM和KOGM作为天然安全的食材,可增加乳糖酶活性,治疗乳糖不耐受症。
The possibility of intestinal prebiotics of konjac glucomannan (KGM) and konjac oligo-glucomannan (KOGM) in treatment of lactose intolerance was studied both in vivo and in vitro. According to the in vitro fermentation of fecal flora from healthy and lactose intolerant subjects, compared to the control, pH value of fermentation broth with KGM and KOGM addition decreased by 1.46-1.56, whereas the output of short-chain fat acids (SCFA) increased significantly (P<0.05). Meanwhile, five weeks old KM mice were given KGM and KOGM for four weeks; the pH value of mice feces decreased while the water content and SCFA concentrations increased significantly (P<0.05). The effect of polysaccharide on lactose fermentation in vitro showed that the pH value in fermentation cultures with KGM and KOGM addition decreased by 2.70-2.83 compared to the control after 24 h. However, the content of acetic acid, butyric acid and lactase activity were increased significantly (P<0.05) by 9.3-17.6 mmol/L, 2.1-2.9 mmol/L and 0.001-0.002 U/mg, respectively. These results indicated that KGM and KOGM could provide a suitable acidic environment that could effectively improve the metabolism of intestinal microflora in mice to produce SCFA. As natural and safe ingredients, KGM and KOGM could increase the activity of lactase, and had a potential usage to treat lactose intolerance.
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