食品与发酵工业 >

2020 , Vol. 46 >Issue 13: 99 - 106

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023044

研究报告

赤霉素缺失型番茄果实抗冷性变化

  • 黄笑非 ,
  • 屈彤彤 ,
  • 丁洋 ,
  • 雷彤彤 ,
  • 张洁 ,
  • 唐选明
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  • 1(中国农业科学院 农产品加工研究所,北京,100193)
    2(北京物资学院,物流学院/北京市物流系统与技术重点实验室,北京,101149)
硕士研究生(唐选明教授为通讯作者,E-mail:tangxuanming@caas.cn)

收稿日期: 2019-12-10

  网络出版日期: 2020-08-04

基金资助

国家重点研发专项(2016YFD0400105)

收起

Chilling resistance of gibberellin-deficiency tomato fruits

  • HUANG Xiaofei ,
  • QU Tongtong ,
  • DING Yang ,
  • LEI Tongtong ,
  • ZHANG Jie ,
  • TANG Xuanming
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  • 1(Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
    2(Beijing Wuzi University, School of Logistics/Beijing Key Laboratory of Logistics System and Technology, Beijing 101149, China)

Received date: 2019-12-10

  Online published: 2020-08-04

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摘要

为明确赤霉素在番茄果实抗冷调控中的作用地位,以绿熟期的野生型番茄果实及其赤霉素缺失型突变株(gib-3)为试材,4 ℃下贮藏,统计贮藏期间番茄果实冷害指数,观察细胞超微结构,测定细胞膜损伤强度、细胞膜脂酶、细胞壁降解酶以及抗氧化酶等活性的变化,研究赤霉素缺失型番茄果实抗冷特性。结果表明,与野生型番茄果实相比,赤霉素缺失型番茄果实冷害症状和超微结构破坏程度更为严重,丙二醛含量、离子渗透率升高,磷脂酶D、脂氧合酶、多聚半乳糖醛酸酶、果胶甲酯酶活性增大,细胞膜过氧化反应和细胞壁降解速度加快,果实细胞膜和细胞壁的完整性受到破坏;超氧化物歧化酶、过氧化氢酶、苯丙氨酸解氨酶和谷胱甘肽转移酶活性均降低,果实清除自由基能力降低,表明低温下赤霉素缺失不利于番茄果实抵御冷害。

关键词： 番茄果实; 赤霉素缺失; 冷害; 抗冷能力

本文引用格式

黄笑非 , 屈彤彤 , 丁洋 , 雷彤彤 , 张洁 , 唐选明 . 赤霉素缺失型番茄果实抗冷性变化[J]. 食品与发酵工业, 2020 , 46(13) : 99 -106 . DOI: 10.13995/j.cnki.11-1802/ts.023044

Abstract

In order to identify the role of gibberellins (GAs) in the resistance of tomato fruits, the present study used the wild type (WT) and GA-deficient mutant (gib-3) tomato fruits at the mature green stage as raw materials and stored them at 4 ℃. The chilling injury (CI) index was measured, the ultra-structure of cells was observed, cell membrane damage intensity, as well as the activity changes of cell membrane lipase, cell wall degradation enzymes, and antioxidant enzymes of tomato fruits were determined. Results indicated that gib-3 tomato fruits showed more severe chilling symptoms and ultra-structural damage than WT tomato fruits. The MDA content, ion permeability and the activity of PLD, LOX, PG and PME of gib-3 tomato were increased. Cell membrane peroxidation and cell wall degradation of gib-3 tomato fruits accelerated, and their integrity of fruit cell membrane and cell wall was damaged. In addition, the activity of SOD, CAT, PAL and GST were decreased in gib-3 tomato fruits, and the ability of free radical scavenging reduced. According to the results, gibberellin deficiency at low temperatures was not good for tomato fruits to resist chilling injury. This study will provide a theoretical basis for the research on the chilling resistance mechanism of postharvest tomato fruits.

Key words： tomato fruit; gibberellin deficiency; chilling injury; cold resistance

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