为明确大黄鱼鱼卵磷脂(large yellow croaker roe phospholipids, LYCRPLs)的乳化姜黄素特性,在单因素试验的基础上,以乳液粒径和稳定性为指标,通过响应面法优化了LYCRPLs对姜黄素的乳化工艺,并采用透射电镜对最优工艺下制备的乳液进行了初步表征,同时,考察了其贮藏稳定性。结果表明,LYCRPLs乳化姜黄素的最优工艺条件为:均质压力72.4 MPa、均质时间141 s、LYCRPLs添加量4.275%、油相比例12.89%,在此条件下所得的乳液粒径为(0.216±0.005 9) μm,乳液颗粒近似于球形,有着明显的水包油结构;为期4周的贮藏稳定性测试显示乳液在5和25 ℃下具有良好的稳定性,无分层和聚集等现象。研究结果为大黄鱼加工副产物的高值化利用提供了理论参考。
In order to verify the emulsifying properties of large yellow croaker roe phospholipids (LYCRPLs), the curcumin was emulsified by LYCRPLs, and the preparation conditions were investigated in terms of emulsion particle size and stability. The emulsifying processing was optimized based on the single factor experiments via response surface methodology. Meanwhile, the emulsion was characterized by transmission electron microscope (TEM). Afterward, the emulsion stability was also investigated. The results showed that the optimum conditions were as follows: homogenization pressure was 724 bar, homogenization time was 141 s, LYCRPLs concentration was 4.275% and oil concentration was 12.89%. Under the above conditions, the emulsions particle size is (0.216±0.0059) μm. The emulsion particles are almost spherical and possess obvious oil-in-water structure. In addition, the LYCRPLs-curcumin emulsion is stable without delamination and aggregation at 5 and 25 ℃ after 4 weeks. The results are meaningful to make high-value utilization of processing by-products of large yellow croaker.
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