从调控蔗糖代谢的角度分析了外源果糖处理减轻雷竹笋(Phyllostachys praecox f. Preveynalis)采后木质化败坏症状的机理。将雷竹笋分别用0(对照)、10、20、30或40 mmol/L的果糖溶液进行喷淋处理(20 ℃),随后置于4 ℃、相对湿度80%~90%条件下冷藏20 d,期间每隔5 d取样测定笋体木质化和品质参数、可溶性糖和尿苷二磷酸葡萄糖(uridine diphosphate glucose,UDPG)含量以及蔗糖代谢和苯丙烷类代谢途径相关基因的表达丰度。结果表明,20 mmol/L果糖处理较其他处理能更为显著地延缓雷竹笋冷藏期间硬度、失重率、笋体褐变度以及木质素和纤维素含量的上升,提高出汁率,维持品质和抗氧化活性,从而有效控制笋体冷藏期间木质化败坏症状的发展。20 mmol/L果糖处理可显著上调雷竹笋在冷藏期间蔗糖合成方向的基因表达量(PpSS2、PpSPS和PpSPP),促进蔗糖合成并减少UDPG的积累;同时,经果糖处理的雷竹笋中苯丙烷类代谢途径相关基因(PpPAL、Pp4CL、PpCAD、PpF5H、PpCCOMT和PpPOD)的表达丰度显著低于对照水平,并伴随着木质素合成量的显著下降。由此推测,果糖处理可通过调控雷竹笋蔗糖代谢途径的方式提高雷竹笋冷藏期间蔗糖合成量,减少木质素合成中间体UDPG的积累,从而竞争性抑制笋体以苯丙烷类代谢途径合成木质素,最终抑制笋体木质化的发生。
The present study aimed to analyze the effects of exogenous fructose treatment on alleviating lignification deterioration of bamboo shoots (Phyllostachys praecox) during cold storage and the involved mechanism on basis of the regulation of sucrose metabolism. The bamboo shoots were sprayed by the fructose solutions at different concentrations (0,10,20,30 or 40 mmol/L) at 20 ℃ and air-dried respectively. Then the bamboo shoots were stored at 4 ℃ for 20 days. During the storage,the bamboo shoots were collected at a 5-day interval for measurement of the lignification,quality parameters,contents of soluble sugars,uridine diphosphate glucose (UDPG) as well as the expressions of a set of relevant genes involved in sucrose and phenylpropanoid metabolism pathway. The results exhibited that the treatment of bamboo shoots with 20 mmol/L fructose could delay the increase of firmness, the loss of weight,browning degree and the contents of lignin and cellulose. And,it could enhance the extractable juice rate and maintain the overall quality and antioxidant activity,thus contributing to the alleviation of lignification deterioration during the cold storage. Meanwhile,the fructose treatment significantly up-regulated the expression levels of PpSS2,PpSPS and PpSPP which could promote the sucrose synthesis and decrease the accumulation of UDPG in bamboo shoots during the storage. On the contrary,the expression levels of PpPAL,Pp4CL,PpCAD,PpF5H,PpCCOMT and PpPOD related to phenylpropanoid metabolism in fructose-treated bamboo shoots were down-regulated remarkably,accompanied with the clear decrease in lignin content. Therefore,it is deduced that the fructose treatment could enhance sucrose synthesis and decrease UDPG accumulation in terms of the substances for lignin synthesis,resulting in competitive inhibition of phenylpropanoid pathway for lignin synthesis and finally inhibiting the lignification deterioration in bamboo shoots during cold storage.
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