食品与发酵工业 >

2010 , Vol. 36 >Issue 03: 144 - 147

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2010.03.015

食品与发酵工业

三波长光度法测定肉制品中总黄酮含量

  • 尉立刚 ,
  • 张生万 ,
  • 齐尚忠 ,
  • 吴菊花 ,
  • 张婵娟 ,
  • 胡永钢
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网络出版日期: 2010-03-25

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Determination of the Total Flavonoids in Meat Product by a Three-wavelength Spectrophotometry

  • Wei Ligang ,
  • Zhang Shengwan ,
  • Qi Shangzhong ,
  • Wu Juhua ,
  • Zhang Chanjuan ,
  • Hu Yonggang
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Online published: 2010-03-25

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摘要

对含有荞麦的新型保健肉制品中总黄酮含量的测定方法进行了系统的研究,建立了一种测定肉制品中总黄酮含量的三波长光度法。结果表明:在选定的测定波长(314、362、409.5nm)下,总黄酮含量在0-50μg/mL内其浓度与吸光度差(δA)呈线性关系,其R2=0.999、RSD=1.21%,回收率为98.90%-105.94%,该法用于实际样品分析取得了令人满意的结果。

关键词： 肉制品; 总黄酮; 三波长; 分光光度法

本文引用格式

尉立刚 , 张生万 , 齐尚忠 , 吴菊花 , 张婵娟 , 胡永钢 . 三波长光度法测定肉制品中总黄酮含量[J]. 食品与发酵工业, 2010 , 36(03) : 144 -147 . DOI: 10.13995/j.cnki.11-1802/ts.2010.03.015

Abstract

The determination of the total Flavonoids in the buckwheat meat product was introduced. Three-wave length spectrophotometry was applied. The results indicated:in the designation determination wavelength(314,362,409.5 nm),the total flavonoids was 0-50 μg/mL,the concentration and absorbent differences were linear. The correlation coefficient R2=0.999,the RSD was 1.21%,the recovery rate was 98.90%-105.94%. The result was reliable in the determination of the samples.

Key words： meat products; total flavonoids; three-wavelength; spectrophotometry

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