以5种粟谷(JG-34、JG-36、77-322、JG-41和SG-1)为试材,在25℃黑暗条件下发芽60h,探讨粟谷发芽期间贮藏物质的动员和γ-氨基丁酸(GABA)含量的变化。结果表明:60h发芽期间,5种粟谷的呼吸强度呈现增长趋势,其中SG-1和JG-34的呼吸强度最高;淀粉含量降低幅度为43%-60%;还原糖和游离氨基酸含量分别增加60%-136%和127%-180%,其中以JG-34中游离氨基酸增量最多;可溶性蛋白含量呈增加趋势。发芽期间粟谷GABA含量得到有效增长,JG-34、77-322、SG-1、JG-41和JG-36中GABA含量分别比发芽前提高2.41、2.23、1.92、2.18和2.61倍,其中JG-34发芽后GABA含量最高,达到135.4μg/g。通过对不同品种粟谷发芽期间GABA富集量的差异分析得出,JG-34是最适合富集GABA的粟谷品种。粟谷发芽期间富集的GABA含量与其芽长、呼吸强度和游离氨基酸含量呈显著正相关。

Mobilization of stored reserves and γ-aminobutyric acid (GABA) during a 60 h germination of five foxtail millet cultivars (JG-34,JG-36,77-322,JG-41 and SG-1) were investigated in this study. The millet were germinated at 25 ℃ in dark incubator. The results showed that during 60 h of germination,respiration rate in five cultivars increased,SG-1 and JG-34 were higher than other cultivars; starch content decreased significantly in the range of 43%-60 %; reducing sugar content and free amino acid content increased in the range of 60%-136 % and 127%-180 %,respectively; soluble protein content increased during all germination periods. There are significant increase of GABA content in five millet cultivars during germination,the increased extent were 1.92-2.61-fold,GABA content in JG-34 had the highest among all cultivars tested,which lead to 13.54 mg/100g. Variance analysis indicated that there were significant difference (P<0.01) in GABA content among all cultivars. JG-34 of five cultivars could be considered as a good source for GABA accumulation. GABA content in millet during germination was significantly positive correlation with sprout length,respiration rate,soluble protein and free amino acid,respectively.