以植物乳酸菌R-02和枯草芽孢杆菌KGC30141为出发菌,采用L18(35)正交试验设计方法,混菌厌氧固态发酵菜籽粕,研究了2株菌接种量、发酵基质中葡萄糖和玉米浆添加比例及环境温度对发酵菜籽粕中硫苷、粗蛋白含量及物料发酵损耗的影响。结果表明,最优发酵条件为:R-02和KGC30141接种量分别为6%和15%,玉米浆添加量20%,葡萄糖添加量3%,环境温度为28℃,料水比1∶1,在此条件下厌氧发酵15d,发酵菜籽粕中的硫苷含量从84μmol/g降到33μmol/g(绝干基础),粗蛋白含量较原料提高了4.15%(绝干基础),物料损耗为3.73%。

The fermented rapeseed meal was produced by mixed strains (Laobacillus plantarum R-02 and Bacillus subtilis KGC30141) through solid state anaerobic fermentation. The effects of the inoculation amount of two strains,the adding proportion of corn-steep liquor and glucose,and environmental temperature on the content of glucosinolates and crude protein,the loss of materials in fermented rapeseed meal were researched and optimized by a L18(35)orthogonal experiment. Results show the optimization conditions as follows:the ratio of inoculation quantity of Laobacillus plantarum and Bacillus subtilis was 6%:15%,corn-steep liquor 20%,glucose 3%,environmental temperature 28℃,material to water ratio 1:1,with the 15-day anaerobic fermentation,the glucosinolates content in the fermented rapeseed meal decreased from 84 μmol/g(d.b.) to 33 μmol/g(d.b.),and the crude protein increased by 4.15% compared to original,the loss of materials was 3.73%.