食品与发酵工业 >

2010 , Vol. 36 >Issue 05: 9 - 12

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2010.05.001

食品与发酵工业

巨大芽孢杆菌(Bacillus megaterium)HN7 β-半乳糖苷酶的耦合固定化及其性质研究

  • 郭志华 ,
  • 赵亮 ,
  • 曹稳根 ,
  • 高贵珍 ,
  • 廖红艳 ,
  • 孔健
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网络出版日期: 2010-05-25

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Study on the Co-immobilization and Properties of Bacillus megaterium β-galactosidase

  • Guo Zhi-hua ,
  • Zhao Liang ,
  • Cao Wen-gen ,
  • Gao Gui-zhen ,
  • Liao Hong-yan ,
  • Kong Jian
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Online published: 2010-05-25

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摘要

采用壳聚糖为载体,以戊二醛为交联剂,通过包埋-交联耦合固定化方法,固定巨大芽孢杆菌(Bacillusm egaterium)HN7β-半乳糖苷酶,研究固定化条件和固定化酶酶学性质。和游离酶相比,以壳聚糖为载体的固定化酶,热稳定性和酸碱稳定性提高。连续催化9批次后,固定化酶酶活仍能达到最初酶活的79%,固定化酶操作稳定性较好。

关键词： β-半乳糖苷酶; 固定化酶; 壳聚糖

本文引用格式

郭志华 , 赵亮 , 曹稳根 , 高贵珍 , 廖红艳 , 孔健 . 巨大芽孢杆菌(Bacillus megaterium)HN7 β-半乳糖苷酶的耦合固定化及其性质研究[J]. 食品与发酵工业, 2010 , 36(05) : 9 -12 . DOI: 10.13995/j.cnki.11-1802/ts.2010.05.001

Abstract

In the study of effects on immobilized Bacillus megaterium HN7 β-galactosidase under different enzyme immobilization conditions and the physicochemical properties of the immobilized enzyme,we used chitosan as a carrier,and deployed embedding-linking co-immobilization method.The immobilized enzyme with the chitosan as the carrier showed high mechanical strength.The enzyme activity reached 79% of its initial activities after nine consecutive reactions,demonstrating the good operational stability of the immobilized enzyme.

Key words： β-galactosidase; immobilized enzyme; chitosan

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