采用单因素试验和L9(34)正交试验设计优化菜粕芥子碱的加速溶剂萃取仪萃取工艺条件。确定了影响菜粕芥子碱得率的主次因素为:上样量、萃取次数、萃取温度和萃取时间,最佳萃取工艺条件为:萃取温度为140℃,静态萃取时间为15 min,萃取次数为3次,上样量为3 g;在最佳工艺条件下,菜粕芥子碱的得率为1.8807%,与热醇回流提取试验相比,得率提高了64.526 3%,经ASE萃取后的菜粕品质也有较大提高,通过红外光谱对提取浓缩液冷冻干燥后的样品分析,证实样品中含有芥子碱特征结构。

Single-factor test and L9(34)orthogonal experimental design were used in the study of Rapeseed-meal-sinapine extraction.Sinapine was measured at 388nm spectrometry,OZT was measured by ISO 5504-1983,TITTO was measured by the total phenols,and protein was determined by Kjeldahl's method.Study showed that the primary and secondary factors were: the sample quality,cycles,extraction temperature and extraction time;The optimum conditions were: extraction temperature 140 ℃,static extraction time 15min,3 times extraction,sample quantity 3g.Under the optimum conditions,the dregs of Sinapine yield was 1.8807 %,which was 64.5263% increasing compared to the hot alcohol extraction.The quality of rapeseed meal after ASE extracted was improved.By infrared spectroscopy analysis,it verified that the eigenstructure of sinapine was existed in concentrated freeze-dried extracts.