对酱油酿造废液中黑色素的提取工艺、抗紫外线活性、清除DPPH自由基的能力以及抗油脂过氧化活性等进行了研究。结果表明:酱油酿造废液中黑色素的提取条件为以体积分数70%乙醇溶液为提取剂,提取温度40℃,提取体系pH3.0~3.5,整个提取过程耗时100 min左右;黑色素对E.coil有光保护作用,具有较强的抗紫外线活性;该黑色素能清除DPPH自由基,当浓度为0.6 mg/mL时,清除DPPH自由基的清除率达到82.10%,比多巴黑色素标准品清除DPPH自由基能力强;该黑色素能延缓油脂酸败,使油脂的存储期延长1倍。

The extraction process of melanin from soy sauce waste,the abilities of anti-UV ray,antioxidative on DPPH Free Radical and anti-peroxide on bean oil were studied.The optimal process parameters were : 70% ethanol as extractive agent,extraction temperature 40℃,pH 3.0~3.5,extraction time 100min.The melanin exhibits the strong ability of anti-UV rays and high inhibition oxidative activity of DPPH Free Radical as well as anti peroxide on bean oil.