研究了甘草多糖的提取及体外抗氧化活性。采用水提醇沉法从宁夏乌拉尔甘草根中提取多糖,以Vc为标样,采用分光光度法测定了多糖溶液的总还原力,羟自由基(·OH)、超氧阴离子(O2-·)、DPPH自由基(DPPH·)的清除作用及抗油脂氧化能力。结果表明:纯度88.03%的宁夏乌拉尔甘草多糖对羟自由基(·OH)、超氧阴离子(O2-·)、DPPH自由基(DPPH·)具有良好的清除能力,同时还具有较强的还原能力和一定的抗油脂氧化能力,活性大小与多糖的浓度呈明显的线性关系。

Extraction and antioxidant activity of polysaccharide from Glycyrrhiza Uralcnsis in Ningxia were studied.The polysaccharide were extracted by aqueous extraction and alcohol precipitation from roots of Glycyrrhiza uralcnsis.Vc was used as control,The antioxidant capacity of the licorice polysaccharide was evaluated by spectrophotometry on its ability of scavenging hydroxyl radical(·OH),superoxide anion radical(O-2·) and 1,1-diphenyl-2-picrylhydrazy radical(DPPH·),total reducing power and the antioxidant capability on oils.Results showed that the scavenging effects of licorice polysaccharide with purity of 88.03% on ·OH,O-2· and DPPH· were significant,the polysaccharide also had a strong total reducing power and the antioxidant capability on oils.All these activities were concentration dependent.