实验以浒苔碱不溶性膳食纤维提取率、膳食纤维颜色为指标,通过正交实验确定了碱不溶性膳食纤维的适宜提取和漂白工艺条件,并初步测定了碱不溶性膳食纤维的功能特性。结果表明:碱不溶性膳食纤维提取的工艺参数为NaOH浓度1 g/L、碱煮时间60 min、HCl浓度1 g/L、酸煮时间60 min,所得膳食纤维提取率为21.97%,颜色是浅绿色。碱不溶性膳食纤维漂白的工艺参数为次氯酸钠溶液浓度0.7 g/L、料液比1∶60(g∶mL)、pH值5、漂白时间45 min,所得膳食纤维产率为16.88%,颜色是类白色;碱不溶性膳食纤维干基含量达70.49%,膨胀力为22.09 mL/g,持水力为510.58%。

The optimum extraction process and bleach process conditions of alkali-insoluble dietary fiber from Enteromorpha prolifera was studied.Color was selected as the indicator of extraction,the optimum extracting was determined by orthogonal test.The functional properties of alkali-insoluble dietary fiber were preliminary determined.The results showed that the optimum extraction process conditions of alkali-insoluble dietary fiber was 1 g/L NaOH,alkaline cooking time 60min,HCl concentration1 g/L,the extraction rate could be as high as 21.97% with the color near light green.The optimum bleach process condition was: 0.7 g/L NaClO,solid-liquid ratio 1∶ 60,pH5,bleach time 45min.The production rates of dietary fiber could be as high as 16.88% with the color near to white.The dry base content,expansive capacity and water holding capacity of the alkali-insoluble dietary fiber from Enteromorpha prolifera were 70.49%.22.09mL/g and 510.58%,respectively.