研究了在玉米芯为主要碳源的液态培养基中,白腐菌-香菇、平菇、灵芝菌株间两两混合培养,在胞外木质素酶系分泌上的互惠性。结果表明:单一菌种培养下,香菇的漆酶(Lac)、木质素过氧化物酶(LiP)和锰过氧化物酶(MnP)活性均于第10天达到峰值,分别为1 120、358.2和875 u/L;平菇的Lac、LiP和MnP活性均于第6天达到峰值,分别为3 060、567.8和1 275 u/L;灵芝的Lac活性于第10天达峰值,为2 945 u/L,LiP和MnP活性于第8天达到峰值,分别为428.2、975 u/L。混菌培养下,香菇+平菇、平菇+灵芝的混合培养体系表现了酶活峰值提高、峰面积增大的优势;其中又属平菇+灵芝组合的效果最明显,体系中Lac、LiP、MnP活性均于第10天达到峰值,分别为11 400、924.5、2 200 u/L。实验表明,不同种属白腐菌株混合培养对体系的木质素酶分泌存在影响。

The species mutualism of lignin-degrading enzymes secretion under co-fermentation in liquid corn-cob medium between white-rot fungi Lentinus edodes,Pleurotus ostreatus and Ganoderma lucidum were studied.The finding demonstrated that: in individual fermentation,the activities of laccase(Lac),lignin peroxidase(LiP) and manganese peroxidase(MnP) of L.edodes all reached the peak values of 1120 u/L,358.2 u/L and 875 u/L in the 10th day;and the activities of Lac,LiP and MnP of P.ostreatus reached the peak values as 3060 u/L,567.8 u/L and 1275 u/L in the 6th day;G.lucidum's Lac active value reached the peak in the 10th day as 2945 u/L,its LiP and MnP active value reached the peak in the 8th day as 428.2 u/L and 975 u/L.Mixed culture of L.edodes and P.ostreatus,P.ostreatus and G.lucidum both can increase the enzymatic active peak value and the peak area.And the co-fermentation of P.ostreatus and G.lucidum has the most obvious effects.In the mixed culture system of P.ostreatus and G.lucidum,the activity of Lac,LiP and MnP all reached the peak values as 11 400 u/L,924.5 u/L and 2200 u/L in the 10th day.The experiment indicated that the mixed culture of different white-rot fungi species had effect on the lignin-degrading enzymes secretion.