考察了不同廉价氮源对A.succinogenes NJ113发酵产酸的影响,结果显示豆饼粉效果较佳。考察A.succi-nogenes NJ113以豆饼粉为氮源发酵制备丁二酸对菌体生长和产酸的影响。结果表明,A.succinogenes NJ113能够利用豆饼粉作氮源发酵制备丁二酸。单独以豆饼粉为氮源时菌体最多能消耗50 g/L初糖。在3 L发酵罐上进行分别以15.53 g/L豆饼粉和10 g/L酵母粉为氮源对A.succinogenes NJ113进行发酵,其中以豆饼粉为氮源时丁二酸产量为35.20 g/L,收率为70.40%,与酵母粉发酵效果相当,副产物甲酸、乙酸浓度分别由9.49 g/L和6.27 g/L降至4.44 g/L和1.14 g/L,乳酸浓度由0.44 g/L增加至2.91 g/L,发酵时间由20 h延长至48 h。以葡萄糖为碳源时,豆饼粉最多能替代6 g/L酵母粉进行发酵,并且最多能消耗100 g/L初糖,丁二酸产量达71.30 g/L。

The effect of some low-cost nitrogen sources on cell growth and succinic acid production by A.succinogenes NJ113 was studied.It was found that the concentration of succinic acid and DCW was the highest when soybean meal was used as nitrogen source by A.succinogenes NJ113.A.succinogenes NJ113 could utilize 50 g/L glucose when 15.53 g/L soybean meal was used as nitrogen source.The production of succinic acid which reached 35.20g/L with soybean meal as nitrogen source was almost the same as fermentation with yeast extract as nitrogen source.Otherwise,the production of acetic acid and formic acid decreased from 9.49 g/L and 6.27 g/L to 4.44 g/L and 1.14 g/L respectively while the concentration of lactic acid increased from 0.44 g/L to 2.91 g/L.The fermentation period prolonged from 20h to 48h.The production of succinic acid reached 71.30 g/L when 6 g/L yeast extract was replaced by soybean meal using 100 g/L glucose as carbon source.