利用点特异性天然同位素分馏核磁共振技术(SNIF-NMR)和同位素比质谱仪(IRMS)技术测定葡萄酒中稳定性同位素D/H、18O/16O和13C/12C的比值,结果表明(D/H)Ⅱ、R、δ18O‰对产地的鉴别提供着主要信息,而(D/H)Ⅰ与δ13C‰提供了较次要的信息,单独使用SNIF-NMR结果只能区别开环境差异很大的地区,加上δ13C‰后,可将温差大的地区区别开,但对环境相似的地区仍无法有效鉴别,最后将(D/H)Ⅱ、R、δ13C‰、δ18O‰同时考虑,对于环境相似的地区也可以有效鉴别。研究结果为中国葡萄酒原产地鉴别提供了一种新的技术手段。

SNIF-NMR and IRMS methods were used to determine the stable isotopic ratios of D / H and 18O/16O of wine.The results showed that methylene(D/H)Ⅱ of ethanol in wine,R andδ18O‰ are the key determinants in identifying the geographical origin of wines whereas methyl(D/H)Ⅰsite andδ13C‰ provide less significant information.Using the results of SNIF-NMR alone can only distinguish areas of considerably different environment.If coupled withδ13C‰,the areas with different temperature can be distinguished,but the areas with similar environment still cannot be separated.If combined the values of(D/H)Ⅰ,(D/H)Ⅱ,R,δ13C‰ andδ18O‰,SNIF-NMR and IRMS methods can differentiate areas with similar environment.The research outcome provides a new technique to determine the geographical origins of Chinese wine.