以富士苹果为试材,研究了鲜切富士苹果在5℃的5%O2+5%CO2或5%O2+10%CO2箱式气调贮藏条件下抗氧化系统的变化情况。每3d测定1次酶促防御系统的酶活性和非酶促防御系统的抗氧化物质的含量,并测定呼吸速率、腐烂率和褐変情况。结果表明:与对照相比,2种CO2浓度的箱式气调贮藏条件均可启动酶促防御系统,使过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性得到提高,同时也加速了非酶促防御系统抗氧化物质的消耗,降低了总酚和Vc含量以及贮藏中后期的还原型谷胱甘肽(GSH)含量。2种浓度的CO2处理可有效减慢呼吸速率,抑制腐烂的发生并有效降低褐变程度,其中5%O2+5%CO2更有利于鲜切富士苹果褐変的控制,而5%O2+10%CO2更有利于抑制腐烂。
In order to determine the effects of plastic box modified atmosphere storage on the antioxidant system of fresh-cut Fuji apple,the condition of 5% O2 +5% CO2 and 5% O2 + 10% CO2 atmosphere at 5℃ were established in this experiment.Meanwhile,the activity of enzymatic defense system,the concentration of non-enzyme antioxidants as well as respiration rate,rot rate and browning degree were measured every 3 d.The results indicated that two kinds of plastic box modified atmosphere with high CO2 were all able to start the enzymatic defense system.Therefore,not only the peroxidase(POD),catalase(CAT) and superoxide dismutase(SOD) activities were increased,but the consumption of antioxidant in non-enzymatic defense system were also accelerate.The total phenolics,Vc and reduced glutathione(GSH) content were decreased.Moreover,5 % O2 + 5 % CO2 and 5 % O2 +10 % CO2 effectively slowed down the respiration rate,inhibited the occurrence of decay and reduced the degree of browning.Apples stored with 5 % O2+5 % CO2 was better in controlling the browning,while 5 % O2 +10 % CO2 was better in inhibition decay.