以柠檬酸三钠-三氯化铁法合成水溶性大豆多糖-铁(Ⅲ)配合物,采用邻菲罗啉分光光度法测得其含铁量为18.1%,表明Fe3+与水溶性大豆多糖基本形成了稳定的配合物,在pH值1～14内不沉淀。对其抗氧化活性的研究表明,水溶性大豆多糖与铁配合后,对羟基自由基、亚硝酸盐的清除活性及对脂质抗氧化的活性均比水溶性大豆多糖好。在浓度为10 mg/mL时,水溶性大豆多糖对羟基自由基的清除率为16.0%,对亚硝酸盐的清除率为53.9%,对脂质体氧化的抑制率为34.9%,而相同条件下水溶性大豆多糖-铁(Ⅲ)配合物对羟基自由基的清除率为29.6%,对亚硝酸盐的清除率为67.5%,对脂质体氧化的抑制率为77.9%。

Soluble soybean polysaccharide-iron(Ⅲ) complex was synthesized by sodium citrate-FeCl3 method.The iron content was 18.1% using phenanthroline spectrophotometry.The results showed that soluble soybean polysaccharide and Fe3+ basically form a stable complex which doesn't precipitate at pH 1~14.The studies of antioxidant activity showed that,after reacting with iron,the hydroxyl radical-scavenging activity,nitrite scavenging activity and lipid peroxidation inhibition of soluble soybean polysaccharide were all getting better.At the concentration of 10mg/mL,hydroxyl radical-scavenging rate,sodium nitrite scavenging rate and lipid peroxidation inhibition rate of soluble soybean polysaccharide were 16.0%,53.9% and 34.9%,while soluble soybean polysaccharide-iron(Ⅲ) complex were 29.6%,67.5% and 77.9%.