以虾仁为研究对象,通过人为模拟在虾仁中接种单核细胞增生李斯特菌后,再用噬菌体ListexTMP100在常温(20℃)、冷藏(4℃、0℃)和冷冻(-18℃)环境下进行处理,分析虾仁中该菌的抑制效果,单核细胞增生李斯特菌采用平板计数法进行计数。结果表明:噬菌体浓度大于2×107PFU/mL时,能有效杀灭虾仁中单核细胞增生李斯特菌(P<0.05);该浓度在20℃下作用虾仁1 h,便能降低1.50 Log10CFU/g(死亡率为99%以上)的单核细胞增生李斯特菌;在0℃和4℃,作用24 h下,单核细胞增生李斯特菌的死亡率达到99.9%;在-18℃下贮藏30d,单核细胞增生李斯特菌在第1天下降了1.38Log10CFU/g。这些结果表明,噬菌体ListexTMP100对虾仁中的单核细胞增生李斯特菌具有明显的抑制效果,可作为一种理想的生物杀菌剂用于水产食品中。

In this paper,shrimp meat infected Listeria monocytogenes was treated by phage ListexTMP100 at room temperature(20℃),cold(4℃,0℃) and frozen(-18℃) in order to investigate the reduction of Listeria monocytogenes in shrimp meat during storage.The population of Listeria monocytogenes in shrimp meat using plate count method.The results showed that phage ListexTMP100 can effectively reduce the population of Listeria monocytogenes in shrimp meat when it's concentration was more than 2×107 PFU/mL(P<0.05).The population of Listeria monocytogenes declined 1.5 log10CFU/g at 20℃ for one hour by treated phage ListexTMP100(2×107pfu/mL).The death rate of Listeria monocytogenes reached 99.9% at 4℃ and 0℃ and the number declined to 1.38Log10CFU/g at-18℃ for 24 hours at the same concentration of phage.These results showed that phage ListexTMP100 is a good biological antibacterial agent against Listeria monocytogenes in shrimp meat which makes it an ideal potential biological agent to improve the safety of fishery products for consumption.