采用紫外线、硫酸二乙酯、紫外线和硫酸二乙酯复合对实验室保藏的1株高产耐热脂肪酶的根霉SFE-L01菌株进行诱变处理,筛选获得1株突变株UD-23,产耐热脂肪酶活力比出发菌株提高了112.39%,达24 000.00 U/mL。对突变株UD-23的产酶条件及部分酶学性质进行了研究,结果表明,突变株UD-23所产脂肪酶最适作用温度为60℃,且具有良好的热稳定性。条件优化后,该突变株产酶酶活达30 000.00 U/mL,比优化前提高了25%。

A high enzyme activity mutant strain UD-23 was selected from a Thermostable Lipase-producing Rhizopus SFE-L01 which was stored in our laboratory by using UV,DES and UV + DES mutagenetic treatments.Compare to the original strain,the mutant's enzyme activity increased by 1.12 times and reached 24 000.00 U/mL.Study of the production conditions and enzymatic characteristics of the lipase from UD-23 showed that the optimal temperature of the lipase from UD-23 was 60℃.After the optimization,the enzyme activity of this mutant strain reached 30 000.00U/mL,increased by 25%.