利用廉价的非粮原料及采用新型高效的固定化发酵技术对于实现丁酸的工业化发酵生产具有重要意义。文中研究了纤维床生物反应器组成形式对固定化酪丁酸梭菌产丁酸过程的影响,结果表明,采用纤维床生物反应器四柱并联发酵时,丁酸终浓度比单柱发酵提高7.19%,尤其是产率提高了136.7%。以木薯渣水解液为碳源,利用纤维床生物反应器四柱并联进行丁酸批次发酵和补料发酵。批次发酵产丁酸浓度达到25.2 g/L,得率0.46 g/g,采用补料发酵,丁酸终浓度可达61.4 g/L。

As butyric acid is an important chemical raw material,using cheap non-grain biomass and FBB(fibrous-bed bioreactor)immobilized fermentation to produce butyric acid has important significance.The investigation showed that the four-column parallel immobilized fermentation technology can greatly improve the production efficiency due to the decrease of fermentation recycle to half.In the fermentation with cassava dregs hydrolysate as substrate,25.2 g/L butyric acid was obtained and the yield was 0.46g/g.At last,the production of butyric acid was increased to 61.4g/L through fed-batch fermentation.