以实验室制备的酵母(1→3)-β-D-葡聚糖为原料,通过酶解法制备水溶性酵母(1→3)-β-D-葡聚糖,并对其结构和生物活性进行了研究。以水溶性酵母葡聚糖得率为指标,通过单因素和正交实验,确定了酵母(1→3)-β-D-葡聚糖酶解的工艺条件。优化后的酶解条件为:酶活浓度0.15 U/mL,底物质量浓度0.5 g/100 mL,酶解温度40℃,pH3.5,酶解时间0.5 h,该酶解条件下水溶性酵母葡聚糖得率为80.3%。红外光谱分析表明,水溶性葡聚糖仍具有(1→3)-β-D-葡聚糖分子构型,刚果红实验表明其具有三螺旋结构。活性实验表明,水溶性葡聚糖能有效提高E.coli诱导的患腹膜炎小鼠的存活率。

The process of preparation water-soluble yeast(1→3)-β-D-glucan with enzymolysis was studied in this paper.Single-factor test and orthogonal test were conducted to study the influences of various factors on the yield of soluble polysaccharide.The optimal reaction conditions:enzyme concentration 0.15 U/mL,substrate concentration 0.5 g/100mL,reaction temperature 40 ℃,pH 3.5 and reaction time 0.5 h.The yield of soluble polysaccharide by these conditions is 80.3%.In order to determine the triple helix structure and the molecular configuration of the samples,Congo Red test and FT-IR spectra were used.The soluble polysaccharide by enzymolysis can stimulate the immune system of mice.Not only can it extend the survival time of the mice which was challenged by E.coli,but also can enhance the survival of it.