已知甲酸可抑制线粒体呼吸链末端的细胞色素氧化酶,低浓度的甲酸可导致细胞内线粒体活性氧的快速爆发并进一步杀死酵母细胞。因此,对可能影响酿酒酵母细胞线粒体产生活性氧的因素进行了研究,分析了菌龄、低温预处理、还原剂、线粒体ATP酶基因突变等对甲酸毒性的影响。结果表明,酿酒酵母对甲酸的抗性与线粒体活性密切相关,通过线粒体突变降低线粒体活性可增强酿酒酵母对甲酸的抗性。

Basing on the discovery that formic acid can inhibit the cytochrome oxidase which is located at the end of mitochondrial respiratory chain,and low concentration of formic acid can lead to the rapid outbreak of the reactive oxygen species which can kill yeast cells further,factors affecting the production of reactive oxygen species in Saccharomyces cerevisiae cells were studied.Effects of cell age,low-temperature,reducing agents pretreatment,and mitochondrial ATPase gene mutation to formic acid toxicity were analyzed.The results show that formic acid resistance of Saccharomyces cerevisiae is closely related to mitochondrial activity,reducing mitochondrial activity by mitochondrial mutation can enhance cellular resistance to formic acid.