采用四因子二次回归旋转组合设计,确定了杨梅渣多酚类化合物的提取工艺,并测定了杨梅渣提取物的抗氧化能力。结果表明:以乙醇为提取溶剂时,在该实验条件内各提取因素对杨梅多酚提取量影响大小的顺序为:温度>时间>液料比>乙醇浓度;其优化条件为:乙醇体积分数48.02%,温度74.07℃,液料比值62.73,时间3.22h;此条件下杨梅多酚提取量的理论值为37.69 mg/g,与验证值(37.58 mg/g)接近。在与Vc浓度相同的条件下,杨梅渣提取物对DPPH·的清除作用强于Vc、对HO·的清除作用略低于Vc、但总抗氧化能力及对超氧阴离子自由基的抑制作用明显低于同浓度Vc。

By using quadratic regression rotational combinational design,the extraction technique of polyphenols from Myrica rubra pomace was established and antioxidation of extract was also evaluated. Results showed that the yield of polyphenols was affected obviously in successive order by temperature,time,ratio of liquor to Myrica rubra pomace and ethanol concentration when ethanol was used as extraction solvent. The optimal conditions of polyphenols extraction were ethanol concentration 48. 02%,temperature 74. 07 ℃,ratio of Myrica rubra pomace to liquor 62. 73,time 3. 22 h. In this conditions,the predicted value of polyphenols yield was 37. 69 mg /g,which was very close in experimental value of 37. 58 mg /g. The scavenging ability of the extract from Myrica rubra pomace to the DPPH free radical was stronger than Vc at the same concentration. The scavenging ability to the hydroxyl radical was a little lower than Vc. However the antioxidative and scavenging ability to superoxide anion free radical were much lower than Vc.