为了测定实验室保藏的5株双歧杆菌体外降胆固醇能力,分别采用邻苯二甲醛法与高效液相色谱法测定培养基中胆固醇,对两种方法的线性、精密度和加标回收率进行了对比。5株双歧杆菌菌株的降胆固醇率在12.34%~28.57%,两种方法分别在0~0.20 mg/mL和0~0.70 mg/mL范围内呈现良好的线性关系,精密度相对标准偏差分别为0.32%(n=5)和0.96%(n=5),加标回收率在99.33%~100.18%。整体比较,两种方法均可用于测定双歧杆菌的降胆固醇能力,不过邻苯二甲醛法更适合降胆固醇双歧杆菌的初筛检测。

In order to measure the degradation rate of cholesterol of five strains in vitro,o-phthalalehpyde( OPA)method and High performance liquid chromatography( HPLC) method were used to determine the cholesterol of the medium respectively. Linearity,precision and recovery between the two methods were compared. The results were as follows:the degradation rate of cholesterol of five strains are among 12. 34% ~ 28. 57%,two methods both had good linearity in the rang of 0 ~ 0. 20 mg /mL and 0 ~ 0. 70 mg /mL respectively,precision of relative standard deviation was 0. 32%( n =5) and 0. 96%( n = 5),the spike recoveries were both between 99. 33% and 100. 18%. Overall comparison indicated that both the OPA method and HPLC method can measure the degradation rate of cholesterol of bifidobacteria.