建立了啤酒酚酸的高效液相色谱分析方法。啤酒样品经Oasis HLB柱净化后,所含的没食子酸等15种酚酸化合物可在ZORBAX ODS柱(4.6 mm×250 mm,5μm)上得到良好分离,流动相为甲醇(0.1%乙酸)/水(1%乙酸),梯度洗脱,流速1.0 mL/min,紫外检测波长280 nm,啤酒样品的加标回收率在90%~118%之间,RSD值均<5%,该方法简便、准确、灵敏度高,可用于啤酒中酚酸含量的测定。

A method for the determination of phenolic acids in beer based on solid-phase extraction( SPE) and high performance liquid chromatography( HPLC) was developed. The beer samples were purified with Oasis HLB solid phase extraction cartridges,and the separation of 15 phenolic acids compounds including gallic acid were performed on a ZORBAX ODS column( 4. 6 mm ×250 mm,5 μm) with the mobile phase of methanol( 0. 1% acetic acid) /water( 0. 1% acetic acid) in a gradient elution mode. The flow rate of the mobile phase was 1. 0 mL/min,and the detection wavelength was280nm. The recoveries were ranged from 90% ~118%,and the relative standard deviations( RSD) were less than 5. 0%.The method was simple,accurate,and sensitive and suitable for the determination of phenolic acids in beer.