在2,3,5-氯化三苯基四氮唑(TTC)染色法及96孔板基础上建立高活力与高产乙醇酵母细胞的同步定量筛选方法。选用体积分数为85%二甲基亚砜作为TTF提取剂,培养液(pH8.0)中TTC添加浓度为0.4%,以96孔板在0%、10%、14%的乙醇浓度下培养27株备选酵母菌株,通过酶标仪测定波长486 nm下TTF吸收值。结果显示TTF吸收值越高,酵母活性越高,并且通过比较酵母活性与其乙醇产量,得出两者在一定程度上呈正相关,即高活性酵母其乙醇产量也较高。共筛得6株具有较高细胞活性的菌株。此方法为高活性、高产乙醇酵母及其他微生物及植物细胞的活性的快速、大量检测提供了一条新途径。

A quantitative screening method for yeast with high activity was established based on the TTC( Triphenyl- tetrazolium chloride) staining method and 96-well plate. The optimal test conditions were 85%( V /V) dimethyl sulfoxide,0. 4% TTC,pH 8,and testing wave length 486nm. This assay was performed in 96-well microtitre plate.Twenty seven strains were cultured in the 96-well microtitre plate with 0%,10%,and 14%( V /V) ethanol. The TTF in cell was detected by microplate reader. Strains with higher TTF absorption values had higher ethanol producing ability. The research presented a novel rapid,high throughput method to screen yeast with high activity and high ethanol yield. As results,6 strains with high TTF absorption value were obtained. This screening method was also very useful to screen all kinds of microorganism and plant cells.