文中概述了藻蓝蛋白近年来分离纯化技术的研究方法及其应用情况,包括多步盐析法、双水相萃取法、盐析结合双水相萃取法、反胶团萃取法、壳聚糖亲和沉淀-活性炭吸附-DEAE Sephadex A-25柱层析法、离子交换色谱与疏水间相互作用色谱结合法,总结了影响藻蓝蛋白分离纯化的因素,分析了藻蓝蛋白分离提纯技术的现有问题并提出了相应对策,还对藻蓝蛋白的应用前景进行了展望。

In this review,technological advances on isolation and purification of phycocyanin and their application were summarized from detailed introduction to the purified methods such as mutli-step salting out,aqueous twophase extraction,salting out combined with aqueous two-phase extraction,reversed micellar extraction,chitosan affinity precipitation-activated carbon adsorption-DEAE Sephadex A-25 column chromatography,combined ion exchange chromatography and hydrophobic interaction chromatography.In addition,factors that influence the effect of these methods were discussed.The present problems related to the isolation and purification were analyzed and future application of phycocyanin was proposed.