以蓖麻粕为原料,以水为提取溶剂,在单因素试验的基础上通过响应面法优化了蓖麻碱的微波辅助提取工艺,并通过滤纸片法初步研究了蓖麻碱的抑菌性。微波辅助提取蓖麻碱的最佳工艺条件为:微波功率408.01 W,提取时间5.21 min,液固比12∶1(mL∶g),在此条件下蓖麻碱的提取率预测值为50.74%,验证实验中蓖麻碱提取率为50.81%,相对偏差为0.14%。蓖麻碱的抑菌性结果表明蓖麻碱对供试菌都具有抑菌作用,对各种菌的抑制效果为:大肠杆菌>淀粉液化芽孢杆菌>金黄色葡萄球菌。

The microwave-assisted extraction of ricinine from castor meal was studied by single factor experiment and response surface methodology.The antibacterial activity of ricinine was also investigated by filter paper method.The optimum extraction parameters of ricinine were as follows:microwave power 408.01 W,extraction time 5.21 min and ratio of liquid to solid 12(mL∶ g).Under the above conditions,the predicated extraction rate was 50.74 %,and the actual extraction rate was 50.81 %.The relative deviation was 0.14 %.There was no significant difference between predictive value and actual value.The antibacterial activity of ricinine showed that ricinine had inhibition capacity to all tested strains,and the inhibition order was:Escherichia coli > Bacillus amyloliquefaciens > Staphyloccus aureus.