食品与发酵工业 >

2014 , Vol. 40 >Issue 11: 27 - 32

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.201411005

食品与发酵工业

1株分离自西沙诺尼果原浆细菌菌株CICC10881的鉴定及生物学特性研究

  • 张欣 ,
  • 刘洋 ,
  • 姚粟 ,
  • 曹艳花 ,
  • 翟磊 ,
  • 苏姣姣 ,
  • 谭望桥 ,
  • 程池
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网络出版日期: 2014-11-25

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Identification and characterization of bacterial strain CICC 10881 isolated from Paracel Noni juice

  • ZHANG Xin ,
  • LIU Yang ,
  • YAO Su ,
  • CAO Yan-hua ,
  • ZHAI Lei ,
  • SU Jiao-jiao ,
  • TAN Wang-qiao ,
  • CHENG Chi
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Online published: 2014-11-25

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摘要

以西沙诺尼发酵果原浆中分离到的优势菌株CICC 10881为研究对象,对该菌株的分类地位及生长条件进行了研究。通过形态学描述、生理生化特征、16S rRNA和16S~23S rRNA基因序列分析,将其鉴定为东方醋杆菌(Acetobacter orientalis),该菌首次在诺尼果原浆中分离获得,也是我国首次分离获得此菌种。液态培养条件(培养基、p H、培养温度)对菌株生长影响的研究表明,其最适培养条件为,MRS培养基、温度30℃、p H 5.5。

关键词： 西沙诺尼; 发酵原浆; CICC 10881; 鉴定; 生长条件

本文引用格式

张欣 , 刘洋 , 姚粟 , 曹艳花 , 翟磊 , 苏姣姣 , 谭望桥 , 程池 . 1株分离自西沙诺尼果原浆细菌菌株CICC10881的鉴定及生物学特性研究[J]. 食品与发酵工业, 2014 , 40(11) : 27 -32 . DOI: 10.13995/j.cnki.11-1802/ts.201411005

Abstract

In this study,the bacterial strain CICC 10881 isolated from Paracel Noni fermented juice was identified and its growth conditions were also investigated. The strain was indentified and characterized via 16 S rRNA and16S-23 S rRNA gene sequences,morphological features,and cultural,physiological and biochemical characteristics.Strain CICC 10881 was identified as Acetobacter orientalis. It is the first time that this specie was isolated from the Paracel Noni juice in China. The optimum culture conditions for CICC 10881 were as follows: MRS medium,temperature 30℃ and p H5. 5. This research will provide foundation for further study.

Key words： Paracel Noni; fermented juice; CICC 10881; identification; culture conditions

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