优化得到低氧胁迫下大豆芽菜富集γ-氨基丁酸(GABA)的最适培养液组分为:Na Cl浓度42.49 mmol/L,VB6浓度62.6μmol/L和Ca Cl2浓度6.21 mmol/L;此条件下,大豆芽菜GABA含量为2.77 mg/g DW。对大豆发芽过程中生理生化指标的分析表明:低氧胁迫条件下大豆芽苗生长受到抑制,呼吸速率低于对照,可溶性蛋白分解速率加快,游离氨基酸等小分子物质积累,谷氨酸脱羧酶(GAD)和二胺氧化酶(DAO)活性升高,GABA累积。

The optimal composition of culture solution for γ-aminobutyric acid( GABA) under hypoxia was Na Cl42. 49 mmol / L,VB662. 6 μmol / L and Ca Cl26. 21 mmol / L. GABA content in soybean sprouts was 2. 77 mg / g DW under this condition. The changes of physiological and biochemical parameters of soybean germinated under hypoxia indicated that the growth and the respiration was inhibited,the consumption of crude protein increased. The free amino acid content increased,the glutamate decarboxylase( GAD) and diamine oxidase( DAO) activity was enhanced,thus GABA content was enriched.