为了研究黄秋葵花体外清除亚硝酸盐和阻断亚硝胺合成的能力,在2种模拟人体胃液条件下,采用分光光度法进行检测,并以Vc为阳性对照。结果表明:随着反应浓度的升高,黄秋葵花的提取液对亚硝酸盐的清除能力和阻断亚硝胺合成能力有明显提高,且不同的反应时间、模拟人体胃液条件下效果有一定差异。在添加胃蛋白酶的模拟人体胃液条件下,5 mg/m L黄秋葵花的提取液,反应10 min对亚硝酸盐的清除率为81.02%,对亚硝胺合成的阻断率为54.24%。与Vc进行对比分析,得出黄秋葵花的提取液具有较强的清除亚硝酸盐和阻断亚硝胺合成的能力。

Under the condition of two kinds of simulated human gastric juice,the capability of eliminating nitrite and blocking nitrosamine synthesis by Okra flower extract were studied and compared with VC. The results showed that with the increase of concentration,the rate of eliminating nitrite and blocking nitrosamine synthesis were increased obviously. The blocking effects were different with different reaction time and different simulated gastric juice. Simulated human gastric juice B at 5 mg / m L concentration reacted for 10 minutes could eliminate the nitrite up to 81. 02%,and blocking nitrosamine synthesis to 54. 24%. Compared with VC,the extract from Okra flower showed stronger ability on eliminating nitrite and blocking nitrosamine synthesis.