采用DPPH法、水杨酸法和邻苯三酚自氧化法研究了不同酒精度女贞子酒提取物对DPPH·、羟自由基(·OH)和超氧阴离子自由基(O2-·)的清除作用,采用铝盐法及HPLC测定女贞子酒中总黄酮及木犀草素和槲皮素的浸出率。结果显示,女贞子酒提取物对DPPH·、·OH和O2-·的清除率随女贞子酒中乙醇含量的升高而增强,最高清除率的为40%乙醇含量的女贞子酒提取物,对DPPH·、·OH和O2-·的EC50值分别为12.75、62.86和15.93 mg/m L。并且女贞子酒中总黄酮、木犀草素和芹菜素的浸出率亦随着女贞子酒乙醇含量的升高而升高,结果显示,40%乙醇含量的女贞子酒中总黄酮、木犀草素和芹菜素的浸出率分别为21.96 mg/g、40.07μg/g和28.99μg/g。

The antioxidant activities of extract from different alcohol level Nvzhenzi wine were determined by DPPH·,salicylic acid and pyrogallol auto-oxidation assay. Colorimetry and HPLC were applied to detect the extraction rates of total flavonoids,luteolin and apigenin in Nvzhenzi wine. Results showed that the scavenging effects on DPPH·,OH·,and O-2· in extracts from different alcohol level Nvzhenzi wine were improved with increasing alcohol level,especially the 40% alcohol Nvzhenzi wine whose EC50 values for DPPH·,OH·,O-2· were 12. 75 mg /m L,62. 86 mg / m L and 15. 93 mg / m L,respectively. Moreover,extraction rates of total flavonoids,luteolin and apigenin in Nvzhenzi wine also were improved with increasing alcohol level. The extraction rates of total flavonoids,luteolin and apigenin in 40% alcohol Nvzhenzi wine were 21. 96 mg / g,40. 07 g / g and 28. 99 g / g respectively,which provided a basis for its health effects.