马铃薯淀粉生产过程中会产生大量含有蛋白质等有机成分的汁水,如果直接排放,其中的蛋白质会被分解,从而产生如尸胺、腐胺、精胺、吲哚及硫化物等具有臭味的物质,造成空气污染。该研究通过筛选获得1株能够降解马铃薯汁水中蛋白质的菌株,并且降解后产生的臭味小于气味阀值,将其用于还田灌溉,有效的进行废物利用。实验经过初筛、复筛分离获得了能够降解酪蛋白质的菌株,并进行马铃薯汁水发酵,通过测定分析挥发性成分,未检测到吲哚、硫化氢等臭味成分,尸胺、腐胺、亚精胺和精胺等物质含量极少,感官评价臭味极低,并可以在15 ℃保持较好发酵活力,通过《细菌学手册》及生理生化鉴定和16S rRNA鉴定,确定为粘质沙雷氏菌(Serratia marcescens),命名为NXYS-S。
During the production of potato starch, juice containing protein and other organic components was produced. If the juice is directly discharged, its protein will be decomposed and produce odorous substances such as cadaverine, putrescine, spermine, indole and sulfide, which will cause air pollution. In this study, a strain degrading protein in the potato starch juice was screened, and the odor produced by degradation was lower than the odor threshold. The degraded potato juice could be used for irrigation, and the waste was utilized effectively. By preliminary screening and re-screening, a strain capable of degrading casein was obtained. The potato juice was fermented with the strain, and then by determination of volatile components, no odor components such as indole and hydrogen sulfide were found. The content of cadaverine, putrescine, spermidine, and spermine was very low. The odor was extremely low by sensory evaluation. And the fermentation activity maintained very well at 15 ℃. Through the Handbook of Bacteriology, physiological and biochemical identification , as well as 16S rRNA identification, this strain was identified as Serratia marcescens and named NXYS-S.
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