食品与发酵工业 >

2021 , Vol. 47 >Issue 8: 256 - 260

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025420

综述与专题评论

等温扩增技术在食源性致病菌检测中的研究进展

  • 董晶 ,
  • 卢鑫 ,
  • 郭威 ,
  • 杨倩 ,
  • 张伟
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  • 1(河北农业大学 食品科技学院,河北 保定,071001)
    2(河北农业大学 理工学院,河北 沧州,061100)
    3(河北农业大学 生命科学学院,河北 保定,071001)
硕士研究生(张伟教授为通讯作者,E-mail:zhangwei631126@163.com)

网络出版日期: 2021-05-20

基金资助

国家自然科学基金(31371772);河北省自然科学基金重点项目(C2019204342);河北省重点研究开发计划(18275501D)

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Research progress on isothermal amplification technology in the detection of foodborne pathogens

  • DONG Jing ,
  • LU Xin ,
  • GUO Wei ,
  • YANG Qian ,
  • ZHANG Wei
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  • 1(College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China)
    2(College of Science and Technology, Hebei Agricultural University, Cangzhou 061100, China)
    3(College of Life Sciences, Hebei Agricultural University, Baoding 071001, China)

Online published: 2021-05-20

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摘要

食源性致病菌污染是影响食品质量安全的重要因素之一,加强食源性致病菌的检测力度对于保证食品安全以及预防食源性疾病至关重要。基于核酸的分子检测技术较传统的培养方法相比,更加快速、灵敏、高效。等温扩增技术以反应条件简单等优点逐渐替代变温扩增技术。该文简述了环介导等温扩增、链替代等温扩增、单引物等温扩增、滚环等温扩增以及跨越式滚环等温扩增5种技术的研究进展及其在食源性致病菌快速检测方面的应用,指出了等温扩增反应存在的共性问题,并提出解决措施。此外,还对这5种等温扩增技术进行比较分析,为食源性致病菌的现场快速检测与筛查提供参考依据。

关键词： 食源性致病菌; 等温扩增技术; 分子检测

本文引用格式

董晶 , 卢鑫 , 郭威 , 杨倩 , 张伟 . 等温扩增技术在食源性致病菌检测中的研究进展[J]. 食品与发酵工业, 2021 , 47(8) : 256 -260 . DOI: 10.13995/j.cnki.11-1802/ts.025420

Abstract

Contamination of foodborne pathogens is one of the important factors affecting food quality and safety. Therefore, strengthening the monitoring of foodborne pathogens is essential to ensure food safety and prevent foodborne diseases. Compared with traditional culture methods, nucleic acid-based molecular detection technology is faster, more sensitive, and more efficient. Isothermal amplification technologies gradually replace variable temperature amplification technologies with the advantages of simple reaction conditions. This paper briefly described the research progress on loop-mediated isothermal amplification, strand displacement amplification, single primer isothermal amplification, rolling circle amplification, and saltatory rolling circle amplification, as well as their applications in rapid detection of foodborne pathogenic bacteria. In addition, this research pointed out the common problems existing in the isothermal amplification reaction, and proposed solutions. Otherwise, the five isothermal amplification techniques were compared, which provide a reference for the rapid on-site detection and screening of foodborne pathogens.

Key words： food-borne pathogens; isothermal expansion technique; molecular detection

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