重庆地区有制作和食用家庭自制泡菜的传统,生花是泡菜盐水的常见现象。为了更深入地认识生花相关的真菌群落结构信息,该研究采用Illumina MiSeq技术对生花(P组)和不生花(NP组)盐水样品的ITS1基因进行测序,解析两组的真菌群落结构及其差异。P组样品中主要的属包括:Pichia(85.22%)、Candida(2.07%)、Aspergillus(1.54%)、Fusarium(1.25%);NP组包括:Pichia (46.04%)、Debaryomyces (21.75%)、Aspergillus (9.57%)、Kazachstania (5.31%)、Simplicillium (2.72%)、Penicillium (1.43%)、Wickerhamomyces (1.32%)、Fusarium (1.15%)、Candida (1.04%)、Schwanniomyces (1.03%)。P组真菌的平均拷贝数几乎是NP组的20倍。Kruskal Wallis检验发现两组的Debaryomyces 和Kazachstania相对丰度存在显著性差异。该研究阐述了与生花现象相关的真菌群落结构信息,有助于防控生花现象。
Preparing and consuming home-made Paocai is a tradition in Chongqing. The pellicle (biofilm) formation in Paocai brines is a common problem during fermentation. In order to study the fungi related to pellicle, Illumina MiSeq sequencing was used to sequence the ITS1 genes of the Paocai brine samples with (P group) and without (NP group) pellicle, the differences in fungal community structures between the two groups were investigated. The major genera of group P included Pichia (85.22%), Candida (2.07%), Aspergillus (1.54%) and Fusarium (1.25%). The major genera of NP group included Pichia (46.04%), Debaryomyces (21.75%), Aspergillus (9.57%), Kazachstania (5.31%), Simplicillium (2.72%), Penicillium (1.43%), Wickerhamomyces (1.32%), Fusarium (1.15%), Candida (1.04%) and Schwanniomyces (1.03%). The average biomass of fungi in group P was almost 20 times than that of group NP. Significant differences were observed in the relative abundance of genus Debaryomyces and Kazachstania by Kruskal Wallis test. This study demonstrates the fungal community structure information related to pellicle phenomenon, which is helpful for preventing and controlling pellicle in home-made Paocai.
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