研究报告

母乳源乳酸菌的低聚糖利用特性研究

  • 王鹏 ,
  • 刚梦萱 ,
  • 张臣臣 ,
  • 严浩东 ,
  • 潘丽娜 ,
  • 康文丽 ,
  • 汪家琦 ,
  • 戴智勇 ,
  • 顾瑞霞 ,
  • 陈霞
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  • 1(扬州大学 江苏省乳品生物技术与安全控制重点实验室,江苏 扬州,225127)
    2(澳优乳业(中国)有限公司澳优食品与营养研究院,湖南 长沙,410000)
    3(江苏省乳业生物工程技术研究中心,江苏 扬州,225004)
    4(扬州大学 益生菌与乳品深加工重点实验室,江苏 扬州,225127)
第一作者:硕士研究生(陈霞教授为通信作者,E-mail:chenxia@yzu.edu.cn)

收稿日期: 2021-10-30

  修回日期: 2021-11-16

  网络出版日期: 2022-06-23

基金资助

扬州市-扬州大学市校合作共建科技创新平台(YZ2020265);湖南省营养健康品工程技术研究中心(创新平台与人才计划)(2019TP2066)

Oligosaccharide utilization characteristics of lactic acid bacteria from breast milk

  • WANG Peng ,
  • GANG Mengxuan ,
  • ZHANG Chenchen ,
  • YAN Haodong ,
  • PAN Lina ,
  • KANG Wenli ,
  • WANG Jiaqi ,
  • DAI Zhiyong ,
  • GU Ruixia ,
  • CHEN Xia
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  • 1(Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou University, Yangzhou 225127, China)
    2(Ausnutria Institute of Food and Nutrition, Ausnutria Dairy (China) Co, Ltd, Changsha 410000, China)
    3(Jiangsu Dairy Biotechnology Engineering Research Center, Yangzhou 225004, China)
    4(Key Laboratory of Probiotics and Dairy Deep Processing of Yangzhou University, Yangzhou 225127, China)

Received date: 2021-10-30

  Revised date: 2021-11-16

  Online published: 2022-06-23

摘要

为探究母乳源乳酸菌对不同低聚糖的利用特性,比较了8株不同种属的乳酸菌在6种低聚糖及复配低聚糖培养基中的生长能力。结果表明,低聚果糖和菊粉对4株副干酪乳杆菌均具有促生长作用(生长量大于葡萄糖);低聚果糖和低聚半乳糖能明显促进植物乳杆菌M113的增长;鼠李糖乳杆菌M53对低聚半乳糖的利用情况优于其他低聚糖;2株双歧杆菌对低聚半乳糖和水苏糖的利用较强。所测母乳源乳酸菌均不能较好利用低聚木糖人乳中常见的2′-岩藻糖基乳糖。8株母乳来源的乳酸菌对低聚糖的利用具有菌株特异性。菌株在混合低聚糖中的生长能力取决于其偏爱低聚糖的含量。该实验结果能够为婴幼儿配方奶粉中益生菌和低聚糖的筛选提供数据支持。

本文引用格式

王鹏 , 刚梦萱 , 张臣臣 , 严浩东 , 潘丽娜 , 康文丽 , 汪家琦 , 戴智勇 , 顾瑞霞 , 陈霞 . 母乳源乳酸菌的低聚糖利用特性研究[J]. 食品与发酵工业, 2022 , 48(11) : 101 -106 . DOI: 10.13995/j.cnki.11-1802/ts.029888

Abstract

In order to explore the utilization of oligosaccharides by lactic acid bacteria from breast milk, the growth ability of eight lactic acid bacteria strains in media with different oligosaccharides was compared. The results showed that fructo-oligosaccharide and inulin could promote the growth of four Lactobacillus paracasei strains (compared with glucose); fructo-oligosaccharide and galacto-oligosaccharide could significantly promote the growth of Lactobacillus plantarum M113; Lactobacillus rhamnosus M53 had better utilization of galacto-oligosaccharides than others; two Bifidobacterium strains had stronger utilization of galacto-oligosaccharide and stachyose. None of the tested lactic acid bacteria could make better use of the common 2'-fucosyllactose in human milk and xylo-oligosaccharide. These lactic acid bacteria had strain specificity in the utilization of oligosaccharides. The growth ability of strains in mixed oligosaccharides depends on the content of their preferred oligosaccharides. The results provide data support for the screening of probiotics and oligosaccharides in infant formula milk powder.

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