食品与发酵工业 >

2022 , Vol. 48 >Issue 11: 206 - 212

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029680

研究报告

当归茎叶精油对四种果蔬采后腐败真菌的抑制活性

  • 乔彩红 ,
  • 李斌山 ,
  • 张忠 ,
  • 毕阳 ,
  • 李子和 ,
  • 朱亚同 ,
  • 武淑娟
展开
  • (甘肃农业大学 食品科学与工程学院,甘肃 兰州,730070)
第一作者:硕士研究生(张忠教授和毕阳教授为共同通信作者,E-mail:foodgau@126.com;beyang62@163.com)

收稿日期: 2021-10-12

  修回日期: 2021-11-01

  网络出版日期: 2022-06-23

基金资助

甘肃省自然科学基金项目(21JR7RA807)

收起

Antifungal activities of essential oil from Angelica sinensis leaf against postharvest pathogenic fungi of fruit and vegetables

  • QIAO Caihong ,
  • LI Binshan ,
  • ZHANG Zhong ,
  • BI Yang ,
  • LI Zihe ,
  • ZHU Yatong ,
  • WU Shujuan
Expand
  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070,China)

Received date: 2021-10-12

  Revised date: 2021-11-01

  Online published: 2022-06-23

Fold

摘要

为实现当归废弃物的资源化利用,探讨当归茎叶精油的化学组分及抑真菌活性,采用二倍稀释法确定了当归茎叶精油对4种常见采后病原真菌[互隔交链孢(Alternaria alternata)、硫色镰刀菌(Fusarium sulphureum)、粉红单端孢(Trichothecium roseum)和扩展青霉(Penicillium expansum)]最低抑菌浓度(minimum inhibitory concentration,MIC)和最低杀菌浓度(minimum fungicidal concentration,MFC),并用滤纸片熏蒸法和雾化熏蒸法分别研究了当归茎叶精油对4种真菌菌丝生长的体外抑制作用和体内条件下对T.roseum的抑制效果。结果表明,当归茎叶精油的提取得率为0.057%。在当归茎叶精油中共鉴定出了51种化合物,2, 4, 5-三甲基苯甲醛(22.7%)含量最高,其次是α-蒎烯(11.1%)、十氢-3-甲基-7-亚甲基-1-(1-甲基乙基)(6.69%)、α-法呢烯(6.44%)、反式-蛇床内酯(5.84%)和β-蒎烯(5.16%)。当归茎叶精油对F.sulphureum、A.alternata和P.expansum的MIC均为2.34 μL/mL,MFC均为9.37 μL/mL,对T.roseum的MIC、MFC分别是1.17、9.37 μL/mL。当归茎叶精油熏蒸处理对4种菌的菌丝生长抑制率从高到低依次为T.roseum、F.sulphureum、A.alternata和P.expansum。体内试验发现当归茎叶精油处理能有效抑制T.roseum在樱桃番茄上的扩展,第7天时60、90 μL/mL当归茎叶精油处理组的病斑直径分别比对照组降低了51%、69%。

关键词： 当归茎叶; 精油; 化学成分; 抑菌活性; 樱桃番茄

本文引用格式

乔彩红 , 李斌山 , 张忠 , 毕阳 , 李子和 , 朱亚同 , 武淑娟 . 当归茎叶精油对四种果蔬采后腐败真菌的抑制活性[J]. 食品与发酵工业, 2022 , 48(11) : 206 -212 . DOI: 10.13995/j.cnki.11-1802/ts.029680

Abstract

Angelica sinensis (Oliv.) Diels is authentic medicinal material of Gansu province. To provide theoretical basis for a comprehensive resource utilization of A. sinensis wastes, the extraction ratio, main chemical component and antifungal activity of the A. sinensis leaf essential oil were studied. Double broth dilution was used to determine the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of the essential oil against four postharvest pathogens (Penicillium expansum, Alternaria alternata, Fusarium sulphureum and Trichothecium roseum). Fumigation method was used to determine antifungal activity against the four fungi in vitro and against T. roseum on cherry tomatoes, in vivo. The results showed that the yield of A. sinensis leaf essential oil was 0.057%. Totally 51 components were identified in the essential oil, among them 2, 4, 5-trimethylbenzaldehyde (22.7%) and α-pinene (11.1%) were the most abundant compounds. The MIC and MFC of the essential oil on F. sulphureum, A. alternata and P. expansum was 2.34 μL/mL and 9.37 μL/mL, respectively. The MIC and MFC of T. roseum were 1.17 and 9.37 μL/mL, respectively. The inhibition order of the essential oil on the mycelial growth of the four fungi was T. roseum, F. sulphureum, A. alternata and P. expansum from the highest to the lowest. The essential oil could inhibit the expansion of the pink-mold rot on cherry tomatoes effectively. On the 7th day, the diameter of disease lesions of the 60 and 90 μL/mL groups decreased by 51% and 69% compared with that of control group, respectively.

Key words： Angelica sinensis leaf; essential oil; chemical profile; antifungal activity; cherry tomatoes

参考文献

[1] 张瑛, 王亚丽, 潘新波.当归历史资源分布本草考证[J].中药材, 2016, 39(8):1 908-1 910.
ZHANG Y, WANG Y L, PAN X B.Textual research on the historical resource distribution of Angelica sinensis[J].Journal of Chinese Medicinal Materials, 2016, 39(8):1 908-1 910.
[2] 宋玉红, 陈艳娥, 杜晓红, 等.当归气味的形成与除味方法[J].中国果菜, 2020, 40(9):61-64.
SONG Y H, CHEN Y E, DU X H, et al.Formation of odors and deodorization methods of Chinese Angelica[J].China Fruit & Vegetable, 2020, 40(9):61-64.
[3] 龚成文, 谢志军, 米永伟, 等.当归栽培研究进展[J].中国中医药科技, 2018, 25(5):772-775.
GONG C W, XIE Z J, MI Y W, et al.Advances in cultivation of Angelica sinensis[J].Chinese Journal of Traditional Medical Science and Technology, 2018, 25(5):772-775.
[4] 赵锐明, 陈垣, 郭凤霞, 等.甘肃岷县野生当归资源分布特点及其与栽培当归生长特性的比较研究[J].草业学报, 2014, 23(2):29-37.
ZHAO R M, CHEN Y, GUO F X, et al.Comparative study on distributed feature of wild Angelica sinensis resources and the difference in growth characteristics with its cultivars native to Min County of Gansu[J].Acta Prataculturae Sinica, 2014, 23(2):29-37.
[5] 罗旭东, 李成义, 李俊岳, 等.当归叶总黄酮提取工艺优化[J].辽宁中医杂志, 2018, 45(7):1 456-1 459.
LUO X D, LI C Y, LI J Y, et al.Optimization of reflux extraction of total flavonoids from leaves of Angelica sinensis(oliv.) Diels[J].Liaoning Journal of Traditional Chinese Medicine, 2018, 45(7):1 456-1 459.
[6] 罗旭东. 当归地上部分鉴定学研究及当归叶茶的研制[D].兰州:甘肃中医药大学, 2018.
LUO X D.Study on identification of the aerial parts of Angelica sinensis and preparation of Angelica leaf tea[D].Lanzhou:Gansu University of Chinese Medicine, 2018.
[7] ALI M, HAROON U, KHIZAR M, et al.Facile single step preparations of Phyto-nanoparticles of iron in Calotropis procera leaf extract to evaluate their antifungal potential against Alternaria alternata[J].Current Plant Biology, 2020, 23:100157.
[8] FARZANEH M, KIANI H, SHARIFI R, et al.Chemical composition and antifungal effects of three species of Satureja (S.hortensis, S.spicigera, and S.khuzistanica) essential oils on the main pathogens of strawberry fruit[J].Postharvest Biology and Technology, 2015, 109:145-151.
[9] WEI Y Z, WEI Y Y, XU F, et al.The combined effects of tea tree oil and hot air treatment on the quality and sensory characteristics and decay of strawberry[J].Postharvest Biology and Technology, 2018, 136:139-144.
[10] ZHU C Y, LEI M Y, ANDARGIE M, et al.Antifungal activity and mechanism of action of tannic acid against Penicillium digitatum[J].Physiological and Molecular Plant Pathology, 2019, 107:46-50.
[11] MAFFEI M E.Sites of synthesis, biochemistry and functional role of plant volatiles[J].South African Journal of Botany, 2010, 76(4):612-631.
[12] 王丹, 张静, 贾晓曼, 等.丁香精油对甜樱桃采后优势致腐真菌的控制及其抑菌机理[J].核农学报, 2020, 34(6):1 221-1 229.
WANG D, ZHANG J, JIA X M, et al.Antifungal activity and possible mechanism of clove essential oil on dominant pathogens of postharvest sweet cherries[J].Journal of Nuclear Agricultural Sciences, 2020, 34(6):1 221-1 229.
[13] JENA S, RAY A, SAHOO A, et al.Deeper insight into the volatile profile of essential oil of two Curcuma species and their antioxidant and antimicrobial activities[J].Industrial Crops and Products, 2020, 155:112830.
[14] YANG X P, JIANG X D.Antifungal activity and mechanism of tea polyphenols against Rhizopus stolonifer[J].Biotechnology Letters, 2015, 37(7):1 463-1 472.
[15] VELÁZQUEZ-NUÑEZ M J, AVILA-SOSA R, PALOU E, et al.Antifungal activity of orange (Citrus sinensis var.Valencia) peel essential oil applied by direct addition or vapor contact[J].Food Control, 2013, 31(1):1-4.
[16] WANG Y, LIU X Y, CHEN T, et al.Antifungal effects of hinokitiol on development of Botrytis cinerea in vitro and in vivo[J].Postharvest Biology and Technology, 2020, 159:111038.
[17] 周桂生, 杨念云, 唐于平, 等.当归地上部分的化学成分及其生物活性研究[J].中国天然药物, 2012, 10(4):295-298.
ZHOU G S, YANG N Y, TANG Y P, et al.Chemical constituents from the aerial parts of Angelica sinensis and their bioactivities[J].Chinese Journal of Natural Medicines, 2012, 10(4):295-298.
[18] GHAVAM M, MANCONI M, MANCA M L, et al.Extraction of essential oil from Dracocephalum kotschyi Boiss.(Lamiaceae), identification of two active compounds and evaluation of the antimicrobial properties[J].Journal of Ethnopharmacology, 2021, 267:113513.
[19] MITHÖFER A, BOLAND W.Plant defense against herbivores:Chemical aspects[J].Annual Review of Plant Biology, 2012, 63:431-450.
[20] JI D C, CHEN T, MA D Y, et al.Inhibitory effects of methyl thujate on mycelial growth of Botrytis cinerea and possible mechanisms[J].Postharvest Biology and Technology, 2018, 142:46-54.
[21] JIAO W X, LIU X, CHEN Q M, et al.Epsilon-poly-L-lysine (ε-PL) exhibits antifungal activity in vivo and in vitro against Botrytis cinerea and mechanism involved[J].Postharvest Biology and Technology, 2020, 168(19):111270.
[22] WANG B, LIU F, LI Q, et al.Antifungal activity of zedoary turmeric oil against Phytophthora capsici through damaging cell membrane[J].Pesticide Biochemistry and Physiology, 2019, 159:59-67.
[23] DA ROCHA NETO A C, NAVARRO B B, CANTON L, et al.Antifungal activity of palmarosa (Cymbopogon martinii), tea tree (Melaleuca alternifolia) and star anise (Illicium verum) essential oils against Penicillium expansum and their mechanisms of action[J].LWT, 2019, 105:385-392.
[24] TAO N G, OUYANG Q L, JIA L.Citral inhibits mycelial growth of Penicillium italicum by a membrane damage mechanism[J].Food Control, 2014, 41:116-121.
[25] TYAGI A K, MALIK A.Antimicrobial potential and chemical composition of Mentha piperita oil in liquid and vapour phase against food spoiling microorganisms[J].Food Control, 2011, 22(11):1 707-1 714.
[26] KACEM N, ROUMY V, DUHAL N, et al.Chemical composition of the essential oil from Algerian Genista quadriflora Munby and determination of its antibacterial and antifungal activities[J].Industrial Crops and Products, 2016, 90:87-93.
[27] XU D, WEI M Q, PENG S R, et al.Cuminaldehyde in cumin essential oils prevents the growth and aflatoxin B1 biosynthesis of Aspergillus flavus in peanuts[J].Food Control, 2021, 125(6):107985.
[28] YANG R P, MIAO J Y, SHEN Y T, et al.Antifungal effect of cinnamaldehyde, eugenol and carvacrol nanoemulsion against Penicillium digitatum and application in postharvest preservation of Citrus fruit[J].LWT, 2021, 141:110924.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司