该文系统研究了不同分子质量和极性溶剂处理的菜籽多肽对β-胡萝卜素包封的影响。采用超滤和不同极性的溶剂对多肽溶液进行处理并分离,得到不同分子质量和两亲性的多肽,然后对β-胡萝卜素进行包封。结果表明,分子质量为1~3 kDa和经75%(体积分数)的乙腈水溶液分离后的菜籽多肽包封β-胡萝卜素效果最佳。经超滤和极性分级联合处理后,多肽的包封率与单独处理组相比提高了6.13%,菜籽多肽与β-胡萝卜素的相互作用进一步加强,使得包封率和负载量有较大程度的提升。此外,复合物聚集情况得到改善,粒径明显变小且分布更加均匀。该研究可为多肽包封β-胡萝卜素提供理论依据。
This paper systematically studied the effects of peptides treated with different molecular weight and polar solvents on the β-carotene encapsulated. In order to obtain peptides with different molecular weight and amphipathic properties, ultrafiltration and solvents of different polarities were used to separate the peptide solutions. Then polypeptide was used to wrap β-carotene. The results showed that the rapeseed polypeptides with molecular weight of 1-3 kDa and separated by 75% acetonitrile aqueous solution had the best encapsulation effect of β-carotene. After combined treatment of ultrafiltration and polarity classification, the encapsulation rate of peptides reached 95.79% compared with the single treatment group. The interaction between rapeseed polypeptide and β-carotene was further strengthened, which greatly improved the encapsulation efficiency and load capacity. Furthermore, the aggregation of the composite was improved, the particle size was significantly smaller and the distribution was more uniform. This study could provide a theoretical basis for the peptide encapsulation of β-carotene.
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