食品与发酵工业 >

2022 , Vol. 48 >Issue 16: 79 - 86

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030277

研究报告

远东拟沙丁鱼黄嘌呤氧化酶抑制肽的制备及其降尿酸活性的研究

  • 詹苏泓 ,
  • 吉宏武 ,
  • 张迪 ,
  • 韦柳益 ,
  • 彭烁 ,
  • 陈铭 ,
  • 宋文奎 ,
  • 瞿瑜杉 ,
  • 刘书成
展开
  • 1(广东海洋大学 食品科技学院,广东 湛江,524088)
    2(广东省水产品加工与安全重点实验室,广东 湛江,524088)
    3(广东省海洋生物制品工程实验室,广东 湛江,524088)
    4(广东省海洋食品工程技术研究中心,广东 湛江,524088)
    5(广东省高等学校水产深加工重点实验室,广东 湛江,524088)
    6(大连理工大学海鲜深加工协同创新中心,辽宁 大连,116034)
硕士研究生(吉宏武教授为通信作者,E-mail:jihw62318@163.com)

收稿日期: 2021-11-30

  修回日期: 2021-12-19

  网络出版日期: 2022-09-16

基金资助

国家重点基础研究发展计划“蓝色粮仓专项”(2019YFD0902004)

收起

Preparation of the xanthine oxidase inhibitory peptide from Sardinops sagax and its activity on reducing uric acid

  • ZHAN Suhong ,
  • JI Hongwu ,
  • ZHANG Di ,
  • WEI Liuyi ,
  • PENG Shuo ,
  • CHEN Ming ,
  • SONG Wenkui ,
  • QU Yushan ,
  • LIU Shucheng
Expand
  • 1(College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China)
    2(Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang 524088, China)
    3(Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China)
    4(Guangdong Provincial Engineering Technology Research Center of Marine Food, Zhanjiang 524088, China)
    5(Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    6(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)

Received date: 2021-11-30

  Revised date: 2021-12-19

  Online published: 2022-09-16

Fold

摘要

为了从远东拟沙丁鱼肉酶解物中制备黄嘌呤氧化酶(xanthine oxidase,XO)抑制肽并研究其降尿酸活性,该研究以远东拟沙丁鱼肉为原料,XO抑制率为考察指标,采用碱性蛋白酶水解远东拟沙丁鱼肉,产生的酶解物通过超滤与凝胶色谱、反相高效液相色谱(reversed phase high performance liquid chromatography,RP-HPLC)分离纯化,从中分离出2个XO抑制肽,它们的氨基酸序列采用超高效液相四级杆飞行时间串联质谱(ultra-high performance liquid chromatography quadrupole time of flight tandem mass spectrometry,UPLC-MS/MS)鉴定为苯丙氨酸-亮氨酸-精氨酸(Phe-Leu-Arg,FLR)与精氨酸-脯氨酸-赖氨酸(Arg-Pro-Lys,RPK)。采用腺苷及XO联合诱导人肾皮质近曲小管上皮细胞(HK-2)构建高尿酸细胞模型,对2个肽的降尿酸功效进行评价,结果显示FLR、RPK具有显著降尿酸作用。

关键词： XO抑制肽; 氨基酸序列; HK-2高尿酸模型; 降尿酸; 远东拟沙丁鱼

本文引用格式

詹苏泓 , 吉宏武 , 张迪 , 韦柳益 , 彭烁 , 陈铭 , 宋文奎 , 瞿瑜杉 , 刘书成 . 远东拟沙丁鱼黄嘌呤氧化酶抑制肽的制备及其降尿酸活性的研究[J]. 食品与发酵工业, 2022 , 48(16) : 79 -86 . DOI: 10.13995/j.cnki.11-1802/ts.030277

Abstract

In order to prepare xanthine oxidase (XO) inhibitory peptides from the hydrolysates of Sardinops sagax, and study their activity of reducing uric acid, the XO inhibition rate was used as the index, S. sagax meat was hydrolyzed by alkaline protease. The hydrolysates obtained were purified by ultrafiltration, gel chromatography and reverse phase high performance liquid chromatography(RP-HPLC), two XO inhibitory peptides were purified. Their amino acid sequences were identified as Phe-Leu-Arg (FLR) and Arg-Pro-Lys (RPK) by ultra-high performance liquid chromatography quadrupole time of flight tandem mass spectrometry (UPLC-MS/MS). A hyperuricemic cell model was established by combined inducing of HK-2 with adenosine and XO. Lowering uric acid effects of these two peptides were evaluated. The results showed that FLR and RPK both had significant effects of lowering uric acid.

Key words： xanthine oxidase inhibitory peptide; amino acid sequence; HK-2 hyperuricemic acid model; reducing uric acid; Sardinops sagax

参考文献

[1] CHEN Y E, LI C T, DUAN S N, et al.Curcumin attenuates potassium oxonate-induced hyperuricemia and kidney inflammation in mice[J].Biomedicine & Pharmacotherapy, 2019, 118:109195.
[2] 王旭, 罗冬平, 茹彦海, 等.从慢性肾脏病角度看高尿酸血症与痛风的指南更新要点[J].中国全科医学, 2021, 24(33):4 191-4 195.
WANG X, LUO D P, RU Y H, et al.Interpretation of the major updates of guidelines regarding hyperuricemia and gout in chronic kidney disease[J].Chinese General Practice, 2021, 24(33):4 191-4 195.
[3] 骆贤亮, 刘滔, 钱忠英, 等..降尿酸肽对高尿酸血症的作用及研究进展[J].食品科学, 2021, 42(5):340-348.
LUO X L, LIU T, QIAN Z Y, et al.Recent progress in research on hyperuricemia and uric acid-lowering peptides[J].Food Science, 2021, 42(5):340-348.
[4] ZHU C, XU Y, LIU Z H, et al.The anti-hyperuricemic effect of epigallocatechin-3-gallate (EGCG) on hyperuricemic mice[J].Biomedicine & Pharmacotherapy, 2018, 97:168-173.
[5] KONG L D, CAI Y, HUANG W W, et al.Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout[J].Journal of Ethnopharmacology, 2000, 73(1-2):199-207.
[6] CHEN R J, CHEN M H, CHEN Y L, et al.Evaluating the urate-lowering effects of different microbial fermented extracts in hyperuricemic models accompanied with a safety study[J].Journal of Food and Drug Analysis, 2017, 25(3):597-606.
[7] OSADA Y, TSUCHIMOTO M, FUKUSHIMA H, et al.Hypouricemic effect of the novel xanthine oxidase inhibitor, TEI-6720, in rodents[J].European Journal of Pharmacology, 1993, 241(2-3):183-188.
[8] LI Y J, KANG X Y, LI Q Y, et al.Anti-hyperuricemic peptides derived from bonito hydrolysates based on in vivo hyperuricemic model and in vitro xanthine oxidase inhibitory activity[J].Peptides, 2018, 107:45-53.
[9] LIU N X, WANG Y, ZENG L, et al.RDP3, a novel antigout peptide derived from water extract of rice[J].Journal of Agricultural and Food Chemistry, 2020, 68(27):7 143-7 151.
[10] COS P, YING L, CALOMME M, et al.Structure-activity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers[J].Journal of Natural Products, 1998, 61(1):71-76.
[11] YANG Q X, WANG Q L, DENG W W, et al.Anti-hyperuricemic and anti-gouty arthritis activities of polysaccharide purified from Lonicera japonica in model rats[J].International Journal of Biological Macromolecules, 2019, 123:801-809.
[12] 吴远彩, 李日美, 申光荣, 等.小肽对凡纳滨对虾生长、抗氧化能力、非特异性免疫及肠道菌群结构的影响[J].广东海洋大学学报, 2021, 41(5):1-9.
WU Y C, LI R M, SHEN G R, et al.Effects of dietary small peptides on growth, antioxidant capacity, nonspecific immunity and ingut microflora structure of Litopenaeus vannamei[J].Journal of Guangdong Ocean University, 2021, 41(5):1-9.
[13] HE W W, SU G W, SUN-WATERHOUSE D, et al.In vivo anti-hyperuricemic and xanthine oxidase inhibitory properties of tuna protein hydrolysates and its isolated fractions[J].Food Chemistry, 2019, 272:453-461.
[14] 邹琳, 杭妙佳, 李阳, 等.鲣鱼黄嘌呤氧化酶抑制肽酶法制备工艺优化[J].浙江大学学报(农业与生命科学版), 2019, 45(5):550-562.
ZOU L, HANG M J, LI Y, et al.Optimization of enzymatic hydrolysis process for preparing xanthine oxidase inhibitory peptides from skipjack tuna[J].Journal of Zhejiang University(Agriculture and Life Sciences), 2019, 45(5):550-562.
[15] 盛周煌. 罗非鱼皮胶原蛋白降尿酸活性肽的研究[D].广州:华南理工大学, 2018.
SHENG Z H.Study on uric acid-reducing peptide of tilapia skin collagen[D].Guangzhou:South China University of Technology, 2018.
[16] MUROTA I, TAGUCHI S, SATO N, et al.Identification of antihyperuricemic peptides in the proteolytic digest of shark cartilage water extract using in vivo activity-guided fractionation[J].Journal of Agricultural and Food Chemistry, 2014, 62(11):2 392-2 397.
[17] LI Q Y, KANG X Y, SHI C C, et al.Moderation of hyperuricemia in rats via consuming walnut protein hydrolysate diet and identification of new antihyperuricemic peptides[J].Food & Function, 2018, 9(1):107-116.
[18] BALDWIN C C. Fao species identification guide for fishery purposes. the living marine resources of the western central Pacific[J]. Copeia, 2003(1):212-214.
[19] 袁学文, 王炎冰.远东拟沙丁鱼低聚肽化学组成及其增强免疫力功能评价[J].食品与发酵工业, 2018, 44(4):104-110.
YUAN X W, WANG Y B.Chemical composition of oligopeptide from Sardinops sagax and its immunoregulatory activity evaluation[J].Food and Fermentation Industries, 2018, 44(4):104-110.
[20] 李亚会, 李积华, 吉宏武, 等.远东拟沙丁鱼抗氧化肽的分离纯化及结构解析[J].中国食品学报, 2021, 21(2):229-238.
LI Y H, LI J H, JI H W, et al.Isolation, purification and structural analysis of antioxidant peptides from Sardinops sagax[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(2):229-238.
[21] LIU N X, WANG Y, YANG M F, et al.New rice-derived short peptide potently alleviated hyperuricemia induced by potassium oxonate in rats[J].Journal of Agricultural and Food Chemistry, 2019, 67(1):220-228.
[22] SMITH J B, THVENON-EMERIC G, SMITH D L, et al.Elucidation of the primary structures of proteins by mass spectrometry[J].Analytical Biochemistry, 1991, 193(1):118-124.
[23] CRAIG A G, KOERBER S C, RIVIER J E, et al.A strategy to assign a series of fragment ions:Investigation of fragment ions involving peptide side chain and backbone cleavage[J].International Journal of Mass Spectrometry and Ion Processes, 1993, 126:137-149.
[24] HOU C L, LIU D, WANG M, et al.Novel xanthine oxidase-based cell model using HK-2 cell for screening antihyperuricemic functional compounds[J].Free Radical Biology and Medicine, 2019, 136:135-145.
[25] 李宇娟. 鲣鱼降尿酸肽的制备分离、结构表征及功效机制研究[D].广州:华南理工大学, 2019.
LI Y J.Preparation, isolation, structural characterization, and the effect mechanism of uric acid-lowering peptides derived from bonito[D].Guangzhou:South China University of Technology, 2019.
[26] KUMAR A, SHALMANOVA L, HAMMAD A, et al.Induction of IL-8(CXCL8) and MCP-1(CCL2) with oxidative stress and its inhibition with N-acetyl cysteine (NAC) in cell culture model using HK-2 cell[J].Transplant Immunology, 2016, 35:40-46.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司